Caputo Antonella, Sparnacci Katia, Ensoli Barbara, Tondelli Luisa
Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Padova, Italy.
Curr Drug Deliv. 2008 Oct;5(4):230-42. doi: 10.2174/156720108785914961.
The use of particulate polymeric carriers holds great promise for the development of effective and affordable DNA and protein subunit vaccines. Rational development of such vaccine formulations requires a detailed understanding of their physico-chemical properties, cell-free and in vitro behaviour, in addition to particle uptake and processing mechanisms to antigen presenting cells capable of stimulating safe and effective immune responses. We here provide an overview on functional polymeric nano- and micro-particles designed for surface adsorption of proteins and DNA antigens currently under investigation for the formulation of new vaccines, including comments on their preparation method, antigen delivery strategy, cell-free and in vitro behaviour. In addition, we focus on their influence in activating antigen-specific humoral and/or cellular immune responses and on their potential for the development of new vaccines.
使用颗粒状聚合物载体对于开发有效且经济实惠的DNA和蛋白质亚单位疫苗具有巨大潜力。合理开发此类疫苗制剂需要详细了解其物理化学性质、无细胞和体外行为，以及颗粒被能够刺激安全有效免疫反应的抗原呈递细胞摄取和加工的机制。我们在此概述了目前正在研究用于新型疫苗制剂的、设计用于蛋白质和DNA抗原表面吸附的功能性聚合物纳米颗粒和微米颗粒，包括对其制备方法、抗原递送策略、无细胞和体外行为的评论。此外，我们重点关注它们在激活抗原特异性体液和/或细胞免疫反应方面的影响以及它们在新型疫苗开发中的潜力。
