人类结肠腺癌中的转录因子PDX-1：一种潜在的肿瘤标志物？
Transcription factor PDX-1 in human colorectal adenocarcinoma: a potential tumor marker?
作者信息
Ballian Nikiforos, Liu Shi-He, Brunicardi Francis-Charles
机构信息
The Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.
出版信息
World J Gastroenterol. 2008 Oct 14;14(38):5823-6. doi: 10.3748/wjg.14.5823.
AIM
To examine the expression of pancreatic duodenal homeobox-1 (PDX-1) transcription factor in human colorectal cancer.
METHODS
RT-PCR, Western blotting, and immuno-histochemistry were performed to determine the expression pattern of transcription factor PDX-1 in primary colorectal tumor, hepatic metastasis, and benign colon tissue from a single patient.
RESULTS
The highest PDX-1 transcription levels were detected in the metastasis material. Lower levels of PDX-1 were found to be present in the primary tumor, while normal colon tissue failed to express detectable levels of PDX-1. Western blot data revealed a PDX-1 expression pattern identical to that of mRNA expression. Immunohistochemistry confirmed high metastasis PDX-1 expression, lower levels in the primary tumor, and the presence of only traces of PDX-1 in normal colon tissue.
CONCLUSION
These data argue for further evaluation of PDX-1 as a biomarker for colorectal cancer.
目的
检测胰腺十二指肠同源盒-1（PDX-1）转录因子在人类结直肠癌中的表达。
方法
采用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和免疫组织化学法，检测一名患者的原发性结直肠癌肿瘤、肝转移灶及良性结肠组织中转录因子PDX-1的表达模式。
结果
在转移灶材料中检测到最高水平的PDX-1转录。原发性肿瘤中PDX-1水平较低，而正常结肠组织未检测到可表达水平的PDX-1。蛋白质免疫印迹数据显示PDX-1的表达模式与mRNA表达模式一致。免疫组织化学证实转移灶中PDX-1高表达，原发性肿瘤中表达较低，正常结肠组织中仅存在痕量的PDX-1。
结论
这些数据支持进一步评估PDX-1作为结直肠癌生物标志物的可能性。
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