Kinoshita A
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1991 Jun;58(2):447-74. doi: 10.5357/koubyou.58.447.
The purpose of this study was to investigate the structure of the matrix in regenerated cementum on surgically denuded root dentin and on acid-demineralized root dentin in dogs. Non-decalcified specimens were examined two to 16 weeks after surgery. The arrangement of the matrix fiber, the fiber component and the crystal deposition of the cementum were studied by light microscopy and electron microscopy. The results were as follows: 1. There were many differences in the structures of regenerated cementum. The differences were dependent on the presence of resorption of the root dentin, existence of lamellar structure, and density of Sharpey's fibers. 2. Regions without surface resorption of dentin exhibited a dense deposition of crystallites and a rare arrangement of collagen fibrils at the cementodentinal junction. 3. Regions of surface resorption of dentin exhibited a layer where dentinal and cemental crystallites were amalgamated, and/or dentinal and cemental matrix fibers were interdigitated at the cementodentinal junction. 4. Regions subjected to artificial demineralization by citric acid exhibited similar structure to that where surface resorption of dentin was observed. 5. It is suggested that the resorption of dentinal surface during the healing process of a surgically created wound in periodontal tissue occurred as an intermediate stage followed by the establishment of a stronger bind by the increase of superficial contact area at the cementodentinal junction.
本研究的目的是调查犬手术去除牙本质的牙根和酸脱矿牙根上再生牙骨质中基质的结构。术后2至16周对未脱钙标本进行检查。通过光学显微镜和电子显微镜研究牙骨质的基质纤维排列、纤维成分和晶体沉积。结果如下：1. 再生牙骨质的结构存在许多差异。这些差异取决于牙根牙本质吸收的存在、板层结构的存在以及沙比纤维的密度。2. 牙本质无表面吸收的区域在牙骨质牙本质界处表现出微晶的密集沉积和胶原纤维的稀少排列。3. 牙本质表面吸收区域在牙骨质牙本质界处表现出一层牙本质和牙骨质微晶融合和/或牙本质和牙骨质基质纤维相互交错的区域。4. 经柠檬酸人工脱矿的区域表现出与观察到牙本质表面吸收的区域相似的结构。5. 提示在牙周组织手术创口愈合过程中，牙本质表面的吸收作为中间阶段发生，随后通过牙骨质牙本质界处表面接触面积的增加建立更强的结合。
