Beier H M, Elger W, Hegele-Hartung C, Mootz U, Beier-Hellwig K
Department of Anatomy and Reproductive Biology, Medical Faculty, RWTH Aachen, Germany.
J Reprod Fertil. 1991 Jul;92(2):511-23. doi: 10.1530/jrf.0.0920511.
We describe a well-established approach for studying the parameters and mechanisms of synchronization or desynchronization between the maternal and embryonic systems before implantation. It is useful for inducing 'delayed secretion' of the endometrium by different endocrine interventions, which dissociate the endometrial transformation from its control by the corpus luteum. The technique has been achieved by means of direct progesterone antagonists which competitively bind to the progesterone receptor and, in turn, inhibit the physiological effects of progesterone. During the luteal phase, secretory protein patterns indicate the receptive stage of the endometrium. Evidence is presented to show that these patterns, analysed by electrophoresis and densitometry, define the time at which an embryo transfer is promising for implantation and establishment of pregnancy.
我们描述了一种成熟的方法，用于研究着床前母体和胚胎系统之间同步或不同步的参数及机制。这对于通过不同的内分泌干预诱导子宫内膜的“延迟分泌”很有用，这种干预可使子宫内膜的转化与其受黄体控制相分离。该技术是通过直接的孕酮拮抗剂实现的，这些拮抗剂竞争性地与孕酮受体结合，进而抑制孕酮的生理作用。在黄体期，分泌蛋白模式表明子宫内膜的接受阶段。有证据表明，通过电泳和光密度测定法分析这些模式，可以确定胚胎移植有望实现着床和妊娠建立的时间。
