Barrows L R, Hardin J W, Dalrymple G V, Gutman P D, Masters C I, Minton K W
Department of Pharmacology and Toxicology, University of Utah, Salt Lake City 84112.
Radiat Res. 1991 Sep;127(3):345-9.
Deinococcus radiodurans genomic DNA, introduced to Escherichia coli in cloning vectors, has been reported to produce radioresistant E. coli that can be selected by gamma irradiation. In this report prior results are reassessed experimentally, and additional studies are presented. Results to date suggest that the acquired radioresistance of E. coli selected by gamma irradiation does not stem from expression of stable plasmid-encoded D. radiodurans sequences, and that acquired radioresistance is not readily transmitted to naive (unirradiated) E. coli by transformation of plasmid recovered from the radioresistant isolates. Several interpretations are discussed.
据报道，在克隆载体中引入大肠杆菌的耐辐射奇异球菌基因组DNA可产生抗辐射的大肠杆菌，这种大肠杆菌可通过伽马射线照射进行筛选。在本报告中，对先前的结果进行了实验重新评估，并给出了更多研究。迄今为止的结果表明，通过伽马射线照射筛选出的大肠杆菌所获得的抗辐射能力并非源于稳定的质粒编码耐辐射奇异球菌序列的表达，并且从抗辐射分离株中回收的质粒转化后，所获得的抗辐射能力不易传递给未接触过辐射的（未照射过的）大肠杆菌。文中讨论了几种解释。
