Lechaire J P, Denefle J P
Centre de Biologie Cellulaire, CNRS, Ivry sur Seine, France.
Tissue Cell. 1991;23(3):307-15. doi: 10.1016/0040-8166(91)90048-x.
In this report, we studied the formation of breaks in the frog dermis during its remodelling at climactic metamorphosis. This remodelling consisted of detachment of the basement lamella collagen from the epidermis. The detached part, called derived collagen, was progressively fractured by breaks. We focused our attention on dermal cell localization during break formation. Firstly, at early climax, dermal cells were localized inside fractures opened in the derived collagen. Secondly, at the later climax, the fractures became breaks, making room for the dermal cells themselves. Thirdly, in derived collagen of the froglet, the well-opened breaks contained elongated dermal cells. At climax, DAB immunoperoxidase staining of fibronectin revealed a granular pattern at the surface of epidermal and dermal cells. Unexpected staining revealed that the dermal breaks contained fibronectin in the form of vertical lines. The foregoing results suggest that the dermal breaks are migratory pathways for dermal cells in derived collagen remodelled at climax.
在本报告中，我们研究了青蛙在变态高峰期重塑过程中真皮层断裂的形成。这种重塑包括基底层胶原蛋白与表皮的分离。分离出的部分称为衍生胶原蛋白，其逐渐被断裂破坏。我们将注意力集中在断裂形成过程中真皮细胞的定位上。首先，在变态高峰期早期，真皮细胞定位在衍生胶原蛋白中形成的裂缝内部。其次，在变态高峰期后期，裂缝变成断裂，为真皮细胞自身腾出空间。第三，在幼蛙的衍生胶原蛋白中，充分张开的断裂处含有细长的真皮细胞。在变态高峰期，用DAB免疫过氧化物酶对纤连蛋白进行染色，在表皮和真皮细胞表面呈现颗粒状模式。意外的染色结果显示，真皮断裂处含有呈垂直线状的纤连蛋白。上述结果表明，真皮断裂是变态高峰期重塑的衍生胶原蛋白中真皮细胞的迁移途径。
