Parsons R H, Schwartz R
Department of Biology and Biophysics Center, Rensselaer Polytechnic Institute, Troy, New York 12180-3590.
Am J Physiol. 1991 Sep;261(3 Pt 2):R686-9. doi: 10.1152/ajpregu.1991.261.3.R686.
Skin samples from the pelvic, pectoral, and back areas of frogs were taken from control (C) animals and from dehydrated animals under three conditions: dehydrated and not exposed to a bathing medium (D), dehydrated live and ventral surface exposed to a bathing medium (DL), and dehydrated with heart stopped and ventral surface exposed to a bathing medium (DHS). The skin concentration of Na+ and K+ of the pelvic patch in the absence of circulation was significantly reduced [DHS 286 +/- 22 microM/mg dry wt (n = 6)] compared with control [C 392 +/- 21 microM/mg dry wt (n = 8)]. However, the pelvic skin concentration was maintained in a frog with an intact circulation [DL 381 +/- 26 microM/mg dry wt (n = 7)] even in the presence of a high pelvic water flow [684 +/- 105 cm3.cm-2.s-1.10(-7) (n = 13)]. The water uptake in the pectoral region [231 +/- 54 cm3.cm-2.s-1.10(-7) (n = 13)] was not high enough to predict a dilution, and none was found. The concentrations were 354 +/- 21 (n = 8), 359 +/- 22 (n = 7), 353 +/- 26 (n = 7), and 373 +/- 45 microM/mg dry wt (n = 6) for C, D, DL, and DHS, respectively. Examination of the Na+ and K+ concentrations separately in the pelvic skin shows that the lower salt content in DHS frogs is mainly due to a loss of Na+.(ABSTRACT TRUNCATED AT 250 WORDS)
从青蛙的骨盆、胸和背部区域采集皮肤样本，样本取自对照（C）动物以及处于三种条件下的脱水动物：脱水且未暴露于浸泡介质（D）、脱水存活且腹面暴露于浸泡介质（DL）、心脏停搏且腹面暴露于浸泡介质（DHS）。与对照[C 392±21微摩尔/毫克干重（n = 8）]相比，在无循环情况下，骨盆区域皮肤中Na⁺和K⁺的浓度显著降低[DHS 286±22微摩尔/毫克干重（n = 6）]。然而，即使存在较高的骨盆水流[684±105立方厘米·厘米⁻²·秒⁻¹·10⁻⁷（n = 13）]，处于完整循环状态的青蛙[DL 381±26微摩尔/毫克干重（n = 7）]的骨盆皮肤浓度仍能维持。胸区域的水吸收量[231±54立方厘米·厘米⁻²·秒⁻¹·10⁻⁷（n = 13）]不足以预测稀释情况，未发现稀释现象。C、D、DL和DHS组的浓度分别为354±21（n = 8）、359±22（n = 7）、353±26（n = 7）和373±45微摩尔/毫克干重（n = 6）。对骨盆皮肤中Na⁺和K⁺浓度分别进行检测表明，DHS组青蛙较低的盐含量主要是由于Na⁺的流失。（摘要截取自250字）
