一种蛋白水解活性的微量测定法。

A microassay for proteolytic activity.

作者信息

Plantner J J

机构信息

Lorand V. Johnson Laboratory for Eye Research, Department of Surgery, Case Western Reserve University, Cleveland, Ohio 44106.

出版信息

Anal Biochem. 1991 May 15;195(1):129-31. doi: 10.1016/0003-2697(91)90307-f.

DOI:10.1016/0003-2697(91)90307-f

PMID:1888008

Abstract

A quantitative procedure for measuring proteolytic activity, utilizing azoalbumin as substrate, has been developed for use in microtiter plates. An enzyme-linked immunosorbent assay reader is used to measure absorbance. The procedure is sensitive, as well as being both rapid and economical. It is particularly convenient for measuring large numbers of samples, such as fractions from column chromatography.

摘要

已开发出一种利用偶氮白蛋白作为底物来测量蛋白水解活性的定量方法，用于微量滴定板。使用酶联免疫吸附测定仪来测量吸光度。该方法灵敏，且快速经济。对于测量大量样品，如柱色谱分离的级分，它特别方便。