Robertson B D, Kwan-Lim G E, Maizels R M
Department of Pure and Applied Biology, Imperial College of Science and Technology, London, United Kingdom.
Anal Biochem. 1988 Jul;172(1):284-7. doi: 10.1016/0003-2697(88)90444-7.
A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By measuring the radioactivity released the assay is able to detect elastase, trypsin, and collagenase in concentrations of 1 ng/ml or less, while the microtiter format permits multiple sample handling and minimizes sample volumes required for analysis.
本文描述了一种灵敏的微孔板检测方法，用于检测多种蛋白水解酶，该方法使用放射性碘标记的明胶作为底物。该技术使用博尔顿-亨特试剂标记底物，然后将其包被在聚氯乙烯微量滴定板的孔中。通过测量释放的放射性，该检测方法能够检测浓度低至1 ng/ml或更低的弹性蛋白酶、胰蛋白酶和胶原酶，而微量滴定板形式允许同时处理多个样品，并将分析所需的样品体积降至最低。
