[Retinoic acid and 12-0-tetradecanoylphorbol-13-acetate (TPA) inducing bipotent differentiation of cultured primary bone marrow cells from leukemia patients].

The in vitro differentiation of cultured primary bone marrow cells from patients with acute promyelocytic leukemia (M3) and chronic myelocytic leukemia (CML) induced by retinoic acid (RA) and TPA was studied. The results indicated that both the M3 and CML bone marrow cells bipotently differentiated into either myeloid or macrophage-monocytic lineage in response to the inducers. On M3 cells the effect of TPA was more potent than RA, and TPA could inhibit the phenotype of myeloid terminal differentiation induced by RA but not vice versa. However, RA could overcome the TPA-induced inhibition of myeloid terminal differentiation of CML cells. These experiments provide a useful model for studying the molecular mechanism of hematopoietic cell differentiation.