Eccleston J F, Moore K J, Brownbridge G G, Webb M R, Lowe P N
Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London, U.K.
Biochem Soc Trans. 1991 Apr;19(2):432-7. doi: 10.1042/bst0190432.
The use of ribose-modified guanine nucleotides and tryptophan mutants of p21ras, neither of which have significant effect on the kinetic mechanism of the p21ras GTPase and the GAP-activated p21ras GTPase, will now allow a detailed kinetic study of how GAP and other regulatory proteins interact with p21ras. This will lead to a better understanding of how the relative concentrations of 'active' p21ras. GTP and 'inactive' p21ras. GDP are regulated in the cell.
使用核糖修饰的鸟嘌呤核苷酸和p21ras的色氨酸突变体，这两者对p21ras GTP酶和GAP激活的p21ras GTP酶的动力学机制均无显著影响，现在将能够对GAP和其他调节蛋白如何与p21ras相互作用进行详细的动力学研究。这将有助于更好地理解细胞中“活性”p21ras·GTP和“非活性”p21ras·GDP的相对浓度是如何被调节的。
