Stone D M, Jacky P B, Prieur D J
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Genome. 1991 Jun;34(3):407-12. doi: 10.1139/g91-062.
Cytogenetic investigations of the domestic dog, Canis familiaris, were performed on the Doberman pinscher and two Boxer dogs. Conventional homogeneously stained and G-banded metaphases from peripheral blood lymphocyte cultures synchronized with amethopterin and bromodeoxyuridine were studied. These procedures permitted the unequivocal identification of all canine chromosomes. A canine chromosome idiogram was constructed on the basis of the G-banding pattern at the haploid 327-band resolution level. The secondary constrictions and tapering of the telomeric regions characteristic of several canine chromosomes are described. Q-, C-, and NOR-banding were also performed and the salient features are described. This karyotype should enhance the value of the canine species in cytogenetic investigations.
对杜宾犬和两只拳师犬进行了家犬（犬属）的细胞遗传学研究。研究了通过氨甲蝶呤和溴脱氧尿苷同步化处理的外周血淋巴细胞培养物中的常规均匀染色和G带中期相。这些方法能够明确鉴定出所有犬类染色体。基于单倍体327带分辨率水平的G带模式构建了犬类染色体核型图。描述了几种犬类染色体特有的次缢痕和端粒区域的逐渐变细情况。还进行了Q带、C带和核仁组织区（NOR）带分析，并描述了其显著特征。这种核型应能提高犬类在细胞遗传学研究中的价值。
