Baruch Y, Amit T, Hertz P, Enat R, Youdim M B, Hochberg Z
Department of Medicine B, Rambam Medical Center, Haifa, Israel.
J Clin Endocrinol Metab. 1991 Oct;73(4):777-80. doi: 10.1210/jcem-73-4-777.
The recently characterized GH-binding protein (GH-BP) has an amino acid sequence identical to the extracellular domain of the GH receptor. Serum GH-BP reflects the amount of GH receptors, and the liver seems to be their main source. To evaluate the effect of liver disease on GH-BP, 52 patients with liver cirrhosis were studied. Serum GH-BP was measured by a binding assay with dextran-coated charcoal separation. Levels of GH-BP were correlated against the clinical state, assessed by Pugh's score. The GH-BP of 31 Pugh's class A patients was 9.7 +/- 0.5%/50 microL serum, and that of 21 Pugh's class B and C patients was 7.2 +/- 0.5%/50 microL serum compared to 11.3 +/- 0.5%/50 microL serum in age-matched controls. GH-BP correlated negatively with Pugh's score and serum bilirubin, and positively with serum albumin. It did not correlate with serum liver enzymes or serum insulin-like growth factor-I. Scatchard analysis of GH binding to the GH-BP revealed similar binding affinities in Pugh's A, B, and C patients and controls. The binding capacity in cirrhosis was significantly lower than that in controls. We conclude that serum GH-BP is controlled mainly by the liver and can provide an additional measure of disease severity in liver cirrhosis.
最近鉴定出的生长激素结合蛋白（GH-BP）的氨基酸序列与生长激素受体的细胞外结构域相同。血清GH-BP反映了生长激素受体的数量，肝脏似乎是其主要来源。为了评估肝脏疾病对GH-BP的影响，对52例肝硬化患者进行了研究。通过葡聚糖包被活性炭分离结合试验测定血清GH-BP。将GH-BP水平与根据Pugh评分评估的临床状态进行相关性分析。31例Pugh A级患者的GH-BP为9.7±0.5%/50μL血清，21例Pugh B级和C级患者的GH-BP为7.2±0.5%/50μL血清，而年龄匹配对照组为11.3±0.5%/50μL血清。GH-BP与Pugh评分和血清胆红素呈负相关，与血清白蛋白呈正相关。它与血清肝酶或血清胰岛素样生长因子-I无相关性。对GH与GH-BP结合的Scatchard分析显示，Pugh A级、B级和C级患者及对照组的结合亲和力相似。肝硬化患者的结合能力明显低于对照组。我们得出结论，血清GH-BP主要受肝脏控制，可作为评估肝硬化疾病严重程度的一项额外指标。
