猫白血病病毒的病毒分离试验
Virus isolation test for feline leukemia virus.
作者信息
de Noronha F, Poco A, Post J E, Rickard C G
出版信息
J Natl Cancer Inst. 1977 Jan;58(1):129-31. doi: 10.1093/jnci/58.1.129.
PMID:189037
Abstract
A method was developed for detecting feline leukemia virus (FeLV) infection in cats by coculturing separated blood lymphocytes and platelets with clone 81 cells carrying a murine sarcoma genome but free of leukemia virus (S+L-). FeLV caused cell transformation that was easily visible 3-12 days after infection. This method is simple, sensitive, and easily read; it has the advantage of directly detecting infectious FeLV rather than viral antigens.
摘要
开发了一种通过将分离的血液淋巴细胞和血小板与携带鼠肉瘤基因组但无白血病病毒（S+L-）的81克隆细胞共培养来检测猫白血病病毒（FeLV）感染的方法。FeLV引起细胞转化，感染后3至12天易于观察到。该方法简单、灵敏且易于判读；具有直接检测感染性FeLV而非病毒抗原的优点。
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