Bertolotto A, Rocca G, Canavese G, Migheli A, Schiffer D
Neurological Clinic II, University of Turin, Italy.
J Neurosci Res. 1991 Jun;29(2):225-34. doi: 10.1002/jnr.490290213.
Chondroitin sulfate proteoglycan (CS-PG) bearing glycosaminoglycan (GAG) chains containing unsulfate (COS) and 6-sulfate (C6S) disaccharides was immunolocalized in rat and human CNS by using monoclonal antibodies (MAb) specific for the two disaccharides. The immunostaining with both MAb was restricted to the periphery of a neuronal subset in rat and human CNS. Double immunofluorescence showed codistribution of the antigens around the same neuronal population. The staining with anti-COS MAb was stronger than with anti-C6S MAb, suggesting that the proteoglycan (PG) contains mainly COS disaccharides. In different rat cortical areas, 40-60/mm2 positive interneurons were found, the visual cortex showing the highest value. In human cortex, positivity was also observed around the soma of some pyramidal cells. In the rat, positive neurons were also localized in deep cerebellar nuclei, reticular nucleus of the thalamus, and other structures of the midbrain and hindbrain. CA3 region of hippocampus and the external layer of pyriform cortex were characterized by positivity of the neuropil. Immunoelectronmicroscopy showed the antigens in the extracellular space around the neuronal soma, the synaptic elements and the cell processes of the neuropil. The neuronal surface of the soma and of the proximal dendrites were positive, but the pre- and postsynaptic membranes and clefts were negative.
通过使用针对两种二糖的单克隆抗体（MAb），对含有非硫酸化（COS）和6-硫酸化（C6S）二糖的糖胺聚糖（GAG）链的硫酸软骨素蛋白聚糖（CS-PG）在大鼠和人类中枢神经系统中进行了免疫定位。两种MAb的免疫染色均局限于大鼠和人类中枢神经系统中一个神经元亚群的周边。双重免疫荧光显示抗原在同一神经元群体周围共分布。抗COS MAb的染色比抗C6S MAb更强，表明蛋白聚糖（PG）主要包含COS二糖。在不同的大鼠皮质区域，发现每平方毫米有40 - 60个阳性中间神经元，视觉皮质中的阳性值最高。在人类皮质中，在一些锥体细胞的胞体周围也观察到阳性。在大鼠中，阳性神经元还定位于小脑深部核团、丘脑网状核以及中脑和后脑的其他结构。海马体的CA3区和梨状皮质外层的神经毡呈阳性。免疫电子显微镜显示抗原存在于神经元胞体周围的细胞外空间、突触元件和神经毡的细胞突起中。胞体和近端树突的神经元表面呈阳性，但突触前膜和突触后膜以及突触间隙呈阴性。
