荧光显微镜中的照明、波长选择和检测
Illumination, wavelength selection, and detection in fluorescence microscopy.
作者信息
Spring K R
机构信息
National Institutes of Health, Laboratory of Kidney and Electrolyte Metabolism, Bethesda, Maryland.
出版信息
Kidney Int Suppl. 1991 Jul;33:S18-22.
The presently available devices for the illumination, changing of wavelengths, and detection of the resultant fluorescence of biological samples viewed in the light microscope have been described and compared. The optimal choice for illumination is a xenon arc lamp with a filter wheel wavelength selector. The optimal choice for an imaging detector is an intensified CCD (charge-coupled-device) camera. These combinations produce the most rapid, stable, and reproducible results when fluorescence measurements are made on living epithelial cells or isolated renal tubules. Techniques for the simultaneous acquisition of fluorescence and differential interference contrast (DIC) images have also been described and compared.
目前已对用于在光学显微镜下观察生物样品的照明、波长改变以及对产生的荧光进行检测的现有设备进行了描述和比较。照明的最佳选择是配备滤光轮波长选择器的氙弧灯。成像探测器的最佳选择是增强型电荷耦合器件（CCD）相机。当对活的上皮细胞或分离的肾小管进行荧光测量时，这些组合能产生最快速、稳定且可重复的结果。同时获取荧光图像和微分干涉对比（DIC）图像的技术也已被描述和比较。
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