聚合酶链反应驱动的DNA-DNA竞争性杂交：一种用于敏感差异克隆的新方法。 - Suppr

Suppr

超能文献

PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning.

作者信息

Lebeau M C, Alvarez-Bolado G, Wahli W, Catsicas S

机构信息

Institut de Biologie Animale, Université de Lausanne, Switzerland.

出版信息

Nucleic Acids Res. 1991 Sep 11;19(17):4778. doi: 10.1093/nar/19.17.4778.

DOI:10.1093/nar/19.17.4778

PMID:1891371

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328732/

Abstract

摘要

相似文献

PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning.聚合酶链反应驱动的DNA-DNA竞争性杂交：一种用于敏感差异克隆的新方法。

Nucleic Acids Res. 1991 Sep 11;19(17):4778. doi: 10.1093/nar/19.17.4778.

Quantification of hepatitis B virus DNA by competitive amplification and hybridization on microplates.通过微孔板上的竞争性扩增和杂交对乙型肝炎病毒DNA进行定量分析。

Biotechniques. 1993 Jul;15(1):134-9.

Hybrid selection of mRNA with biotinylated DNA.

Genet Anal Tech Appl. 1990 Jun;7(4):80-6. doi: 10.1016/0735-0651(90)90032-b.

Isolation of full-length cDNA clones using SMART cDNA and a biotin-streptavidin bead system.利用SMART cDNA和生物素-链霉亲和素磁珠系统分离全长cDNA克隆。

Biotechniques. 2000 Sep;29(3):444-6, 448, 450. doi: 10.2144/00293bm08.

Simple and efficient cloning of small polymerase chain reaction-generated DNA products.

Anal Biochem. 2000 Oct 15;285(2):278-80. doi: 10.1006/abio.2000.4762.

Application of a subtraction hybridization technique involving photoactivatable biotin and organic extraction to solution hybridization analysis of genomic DNA.

Anal Biochem. 1990 May 1;186(2):369-73. doi: 10.1016/0003-2697(90)90096-r.

Combination of cross-species RNA solution hybridization and immunoprecipitation aids in the cloning of RT-PCR products.跨物种RNA溶液杂交与免疫沉淀相结合有助于RT-PCR产物的克隆。

Biotechniques. 1999 Apr;26(4):616-8. doi: 10.2144/99264bm04.

Homology cloning of cDNA or genomic DNA.

Curr Protoc Neurosci. 2001 May;Chapter 4:Unit 4.1. doi: 10.1002/0471142301.ns0401s00.

Quantitation of PCR products with chemiluminescence.采用化学发光法定量聚合酶链反应产物。

Biotechniques. 1995 May;18(5):908-13.

Cloning differentially expressed genes by using differential display and subtractive hybridization.利用差异显示和消减杂交技术克隆差异表达基因。

Methods Mol Biol. 1997;85:45-68. doi: 10.1385/0-89603-489-5:45.

引用本文的文献

Change of cyclin D2 mRNA expression during murine testis development detected by fragmented cDNA subtraction method.应用片段化cDNA消减杂交法检测小鼠睾丸发育过程中细胞周期蛋白D2 mRNA表达的变化

Dev Growth Differ. 1996 Apr;38(2):141-151. doi: 10.1046/j.1440-169X.1996.t01-1-00003.x.

Multifunctional electrochemical biosensor with "tetrahedral tripods" assisted multiple tandem hairpins assembly for ultra-sensitive detection of target DNA.具有“四面体三脚架”辅助多个串联发夹组装的多功能电化学生物传感器用于超灵敏检测目标DNA。

RSC Adv. 2021 Jun 4;11(33):20046-20056. doi: 10.1039/d1ra02424h. eCollection 2021 Jun 3.

FttA is a CPSF73 homologue that terminates transcription in Archaea.FttA 是一种 CPSF73 同源物，可终止古菌中的转录。

Nat Microbiol. 2020 Apr;5(4):545-553. doi: 10.1038/s41564-020-0667-3. Epub 2020 Feb 24.

The phosphoprotein stathmin is essential for nerve growth factor-stimulated differentiation.磷蛋白18对神经生长因子刺激的分化至关重要。

J Cell Biol. 1996 Jun;133(6):1383-90. doi: 10.1083/jcb.133.6.1383.

Differential cDNA cloning by enzymatic degrading subtraction (EDS).通过酶促降解消减进行差异cDNA克隆（EDS）。

Nucleic Acids Res. 1994 Oct 25;22(21):4381-5. doi: 10.1093/nar/22.21.4381.

Efficient high-resolution genetic mapping of mouse interspersed repetitive sequence PCR products, toward integrated genetic and physical mapping of the mouse genome.小鼠散布重复序列PCR产物的高效高分辨率遗传图谱绘制，迈向小鼠基因组的整合遗传图谱和物理图谱绘制。

Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5302-6. doi: 10.1073/pnas.92.12.5302.

Isolation of DNA sequences deleted in lung cancer by genomic difference cloning.通过基因组差异克隆分离肺癌中缺失的DNA序列。

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9705-9. doi: 10.1073/pnas.89.20.9705.

本文引用的文献

Identification of a photoreceptor-specific mRNA encoded by the gene responsible for retinal degeneration slow (rds).鉴定由导致视网膜变性慢（rds）的基因编码的光感受器特异性mRNA。

Nature. 1989 Mar 2;338(6210):70-3. doi: 10.1038/338070a0.

Phenol emulsion-enhanced DNA-driven subtractive cDNA cloning: isolation of low-abundance monkey cortex-specific mRNAs.苯酚乳液增强的DNA驱动消减cDNA克隆：低丰度猴皮层特异性mRNA的分离

Proc Natl Acad Sci U S A. 1988 Mar;85(5):1696-700. doi: 10.1073/pnas.85.5.1696.

A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction.一种采用光活化生物素和苯酚抽提的简单消减杂交技术。

Nucleic Acids Res. 1988 Nov 25;16(22):10937. doi: 10.1093/nar/16.22.10937.

Brain-specific genes: strategies and issues.脑特异性基因：策略与问题。

Curr Top Dev Biol. 1987;21:117-50. doi: 10.1016/s0070-2153(08)60135-0.

Developmentally regulated rat brain mRNAs: molecular and anatomical characterization.发育调控的大鼠脑信使核糖核酸：分子与解剖学特征

J Neurosci. 1987 Aug;7(8):2433-44.

A method for difference cloning: gene amplification following subtractive hybridization.一种差异克隆方法：消减杂交后的基因扩增。

Proc Natl Acad Sci U S A. 1990 Apr;87(7):2720-4. doi: 10.1073/pnas.87.7.2720.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验