Di Paolo G, Pellier V, Catsicas M, Antonsson B, Catsicas S, Grenningloh G
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
J Cell Biol. 1996 Jun;133(6):1383-90. doi: 10.1083/jcb.133.6.1383.
Stathmin is a ubiquitous cytosolic protein which undergoes extensive phosphorylation in response to a variety of external signals. It is highly abundant in developing neurons. The use of antisense oligonucleotides which selectively block stathmin expression has allowed us to study directly its role in rat PC12 cells. We show that stathmin depletion prevents nerve growth factor (NGF)-stimulated differentiation of PC12 cells into sympathetic-like neurons although the expression of several NGF-inducible genes was not affected. Furthermore, we found that stathmin phosphorylation in PC12 cells which is induced by NGF depends on mitogen-activated protein kinase (MAPK) activity. We conclude that stathmin is an essential component of the NGF-induced MAPK signaling pathway and performs a key role during differentiation of developing neurons.
Stathmin是一种普遍存在的胞质蛋白，它会响应各种外部信号而发生广泛的磷酸化。它在发育中的神经元中高度丰富。使用选择性阻断stathmin表达的反义寡核苷酸使我们能够直接研究其在大鼠PC12细胞中的作用。我们发现，尽管几种NGF诱导基因的表达未受影响，但stathmin缺失可阻止神经生长因子（NGF）刺激的PC12细胞分化为交感样神经元。此外，我们发现由NGF诱导的PC12细胞中的stathmin磷酸化依赖于丝裂原活化蛋白激酶（MAPK）活性。我们得出结论，stathmin是NGF诱导的MAPK信号通路的重要组成部分，并且在发育中神经元的分化过程中起关键作用。
