磷蛋白18对神经生长因子刺激的分化至关重要。
The phosphoprotein stathmin is essential for nerve growth factor-stimulated differentiation.
作者信息
Di Paolo G, Pellier V, Catsicas M, Antonsson B, Catsicas S, Grenningloh G
机构信息
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
出版信息
J Cell Biol. 1996 Jun;133(6):1383-90. doi: 10.1083/jcb.133.6.1383.
Abstract
Stathmin is a ubiquitous cytosolic protein which undergoes extensive phosphorylation in response to a variety of external signals. It is highly abundant in developing neurons. The use of antisense oligonucleotides which selectively block stathmin expression has allowed us to study directly its role in rat PC12 cells. We show that stathmin depletion prevents nerve growth factor (NGF)-stimulated differentiation of PC12 cells into sympathetic-like neurons although the expression of several NGF-inducible genes was not affected. Furthermore, we found that stathmin phosphorylation in PC12 cells which is induced by NGF depends on mitogen-activated protein kinase (MAPK) activity. We conclude that stathmin is an essential component of the NGF-induced MAPK signaling pathway and performs a key role during differentiation of developing neurons.
摘要
Stathmin是一种普遍存在的胞质蛋白，它会响应各种外部信号而发生广泛的磷酸化。它在发育中的神经元中高度丰富。使用选择性阻断stathmin表达的反义寡核苷酸使我们能够直接研究其在大鼠PC12细胞中的作用。我们发现，尽管几种NGF诱导基因的表达未受影响，但stathmin缺失可阻止神经生长因子（NGF）刺激的PC12细胞分化为交感样神经元。此外，我们发现由NGF诱导的PC12细胞中的stathmin磷酸化依赖于丝裂原活化蛋白激酶（MAPK）活性。我们得出结论，stathmin是NGF诱导的MAPK信号通路的重要组成部分，并且在发育中神经元的分化过程中起关键作用。
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本文引用的文献
1
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.鉴定一种与微管蛋白二聚体相互作用并提高微管解聚速率的蛋白质。
Cell. 1996 Feb 23;84(4):623-31. doi: 10.1016/s0092-8674(00)81037-5.
2
A branched signaling pathway for nerve growth factor is revealed by Src-, Ras-, and Raf-mediated gene inductions.Src、Ras和Raf介导的基因诱导揭示了神经生长因子的分支信号通路。
Mol Cell Biol. 1993 Jun;13(6):3146-55. doi: 10.1128/mcb.13.6.3146-3155.1993.
3
Signal transduction pathways in neuronal differentiation.
Curr Opin Neurobiol. 1993 Feb;3(1):14-9. doi: 10.1016/0959-4388(93)90029-x.
4
Developmental mechanisms that generate precise patterns of neuronal connectivity.产生精确神经元连接模式的发育机制。
Cell. 1993 Jan;72 Suppl:77-98. doi: 10.1016/s0092-8674(05)80030-3.
5
6
Serine 25 of oncoprotein 18 is a major cytosolic target for the mitogen-activated protein kinase.癌蛋白18的丝氨酸25是丝裂原活化蛋白激酶的主要胞质靶点。
J Biol Chem. 1993 Jul 15;268(20):15039-47.
7
The mitogen-activated protein kinase signal transduction pathway.丝裂原活化蛋白激酶信号转导通路。
J Biol Chem. 1993 Jul 15;268(20):14553-6.
8
Stathmin: cellular localization of a major phosphoprotein in the adult rat and human CNS.微管相关蛋白2：成年大鼠和人类中枢神经系统中一种主要磷蛋白的细胞定位。
J Comp Neurol. 1993 Nov 22;337(4):655-68. doi: 10.1002/cne.903370410.
9
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10
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