Presser S C, Stanczyk F Z, Lobo R A
Department of Obstetrics and Gynecology, University of Southern California School of Medicine, Los Angeles.
Am J Obstet Gynecol. 1991 Sep;165(3):647-51. doi: 10.1016/0002-9378(91)90301-7.
The balance between prostacyclin and thromboxane is known to be important for reproductive and cardiovascular health but has been difficult to quantify. Whereas their stable urinary metabolites, 6-keto-prostaglandin F1 alpha and thromboxane B2, may reflect in part systemic changes, it has been suggested that 2,3-dinor-6-keto-prostaglandin F1 alpha and 11-dehydro-thromboxane B2 more adequately reflect systemic production. Therefore we report for the first time the simultaneous measurements of 6-keto-prostaglandin F1 alpha, thromboxane B2, 2,3-dinor-6-keto-prostaglandin F1 alpha, and 11-dehydro-thromboxane B2 during the menstrual cycle. Timed urine collections were obtained from 18 ovulatory women, aged 22 to 40, during the midfollicular and midluteal phases. Serum estradiol, progesterone, urinary pregnanediol glucuronide, estrone glucuronide, and creatinine also were measured. Prostanoid extraction from urine by C18 and silica Bond Elut columns were then separated by high-performance liquid chromatography before radioimmunoassay. Concentrations of all urinary prostanoids were not significantly different in the follicular phase when compared with the luteal phase. A positive correlation of borderline significance was noted between luteal thromboxane B2 and pregnanediol glucuronide (r = 0.70) and between luteal estrone glucuronide and 2,3-dinor-6-keto-prostaglandin F1 alpha (r = 0.68). A significant correlation was found between follicular estrone glucuronide and the 6-keto-prostaglandin F1 alpha/11-dehydro-thromboxane B2 ratio (r = 0.83, p less than 0.04). These novel normative data suggest an influence of sex steroids on prostacyclin and thromboxane metabolism.
前列环素和血栓素之间的平衡对生殖和心血管健康很重要，这一点已为人所知，但一直难以量化。虽然它们稳定的尿液代谢产物6-酮-前列腺素F1α和血栓素B2可能部分反映全身变化，但有人提出2,3-二去甲-6-酮-前列腺素F1α和11-脱氢-血栓素B2能更充分地反映全身生成情况。因此，我们首次报告了在月经周期中同时测量6-酮-前列腺素F1α、血栓素B2、2,3-二去甲-6-酮-前列腺素F1α和11-脱氢-血栓素B2的情况。从18名年龄在22至40岁的排卵女性中，在卵泡中期和黄体中期进行定时尿液收集。还测量了血清雌二醇、孕酮、尿孕二醇葡萄糖醛酸苷、雌酮葡萄糖醛酸苷和肌酐。通过C18和硅胶键合硅胶柱从尿液中提取前列腺素，然后在放射免疫分析之前通过高效液相色谱进行分离。与黄体期相比，卵泡期所有尿液前列腺素的浓度没有显著差异。黄体期血栓素B2与孕二醇葡萄糖醛酸苷之间（r = 0.70）以及黄体期雌酮葡萄糖醛酸苷与2,3-二去甲-6-酮-前列腺素F1α之间（r = 0.68）存在临界显著的正相关。在卵泡期雌酮葡萄糖醛酸苷与6-酮-前列腺素F1α/11-脱氢-血栓素B2比值之间发现显著相关性（r = 0.83，p < 0.04）。这些新的标准数据表明性类固醇对前列环素和血栓素代谢有影响。
