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马拉维和英国青少年中初始T细胞与记忆T细胞表型的差异:巨细胞病毒的作用?

Differences between naive and memory T cell phenotype in Malawian and UK adolescents: a role for Cytomegalovirus?

作者信息

Ben-Smith Anne, Gorak-Stolinska Patricia, Floyd Sian, Weir Rosemary E, Lalor Maeve K, Mvula Hazzie, Crampin Amelia C, Wallace Diana, Beverley Peter Cl, Fine Paul Em, Dockrell Hazel M

机构信息

Department of Infectious and Tropical Diseases, Keppel Street, London WC1E7HT, UK.

出版信息

BMC Infect Dis. 2008 Oct 15;8:139. doi: 10.1186/1471-2334-8-139.

Abstract

BACKGROUND

Differences in degree of environmental exposure to antigens in early life have been hypothesized to lead to differences in immune status in individuals from different populations, which may have implications for immune responses in later years.

METHODS

Venous blood from HIV-negative adolescents and blood from the umbilical cords of babies, born to HIV-negative women, post-delivery was collected and analysed using flow cytometry. T cell phenotype was determined from peripheral blood lymphocytes and cytomegalovirus (CMV) seropositivity was assessed by ELISA in adolescents.

RESULTS

HIV-negative Malawian adolescents were shown to have a lower percentage of naïve T cells (CD45RO-CD62Lhi CD11alo), a higher proportion of memory T cells and a higher percentage of CD28- memory (CD28-CD45RO+) T cells compared to age-matched UK adolescents. Malawian adolescents also had a lower percentage of central memory (CD45RA-CCR7+) T cells and a higher percentage of stable memory (CD45RA+CCR7-) T cells than UK adolescents. All of the adolescents tested in Malawi were seropositive for CMV (59/59), compared to 21/58 (36%) of UK adolescents. CMV seropositivity in the UK was associated with a reduced percentage of naïve T cells and an increased percentage of CD28- memory T cells in the periphery. No differences in the proportions of naïve and memory T cell populations were observed in cord blood samples from the two sites.

CONCLUSION

It is likely that these differences between Malawian and UK adolescents reflect a greater natural exposure to various infections, including CMV, in the African environment and may imply differences in the ability of these populations to induce and maintain immunological memory to vaccines and natural infections.

摘要

背景

据推测,早年生活中抗原环境暴露程度的差异会导致不同人群个体免疫状态的差异,这可能对晚年的免疫反应产生影响。

方法

采集HIV阴性青少年的静脉血以及HIV阴性女性分娩后婴儿的脐带血,采用流式细胞术进行分析。通过外周血淋巴细胞确定T细胞表型,并采用酶联免疫吸附测定法(ELISA)评估青少年的巨细胞病毒(CMV)血清阳性情况。

结果

与年龄匹配的英国青少年相比,HIV阴性的马拉维青少年显示出较低比例的初始T细胞(CD45RO-CD62Lhi CD11alo)、较高比例的记忆T细胞以及较高比例的CD28阴性记忆(CD28-CD45RO+)T细胞。马拉维青少年的中枢记忆(CD45RA-CCR7+)T细胞比例也低于英国青少年,而稳定记忆(CD45RA+CCR7-)T细胞比例则高于英国青少年。在马拉维接受检测的所有青少年CMV血清均呈阳性(59/59),相比之下,英国青少年为21/58(36%)呈阳性。在英国,CMV血清阳性与外周血中初始T细胞比例降低以及CD28阴性记忆T细胞比例增加相关。在两个地点采集的脐带血样本中,未观察到初始和记忆T细胞群体比例的差异。

结论

马拉维和英国青少年之间的这些差异可能反映出非洲环境中自然接触包括CMV在内的各种感染的情况更为普遍,这可能意味着这些人群在诱导和维持针对疫苗及自然感染的免疫记忆能力方面存在差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cd6/2596152/a0e96616fb69/1471-2334-8-139-1.jpg

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