Hahnel Sebastian, Rosentritt Martin, Bürgers Ralf, Handel Gerhard
Department of Prosthetic Dentistry, Regensburg University Medical Center, Regensburg, Germany.
J Prosthet Dent. 2008 Oct;100(4):309-15. doi: 10.1016/S0022-3913(08)60212-7.
Plaque on dentures may foster the occurrence of denture stomatitis and periodontal diseases in gingival tissues adjacent to partial dentures. Thus, it is beneficial for dental materials to have a low susceptibility to plaque adhesion.
The purpose of this study was to evaluate the susceptibility of commonly used artificial teeth to adhesion of the oral bacterium Streptococcus mutans.
Fifteen specimens each of 12 different artificial teeth were prepared by cutting standardized slabs from the buccal tooth surfaces. After normalizing size (round specimens, diameter of 5 mm, 2 mm thick), polishing (grinding paper, grain 1000 and 4000; universal polishing paste), and assessing surface roughness with a profilometric contact surface measurement device, specimens were incubated with Streptococcus mutans NCTC 10449 suspension for 2.5 hours at 37 degrees C. A veneering composite resin (Sinfony) was used as a control. Adherent bacteria were quantified using a fluorometric assay (Resazurin reduction); relative fluorescence intensity correlates linearly with the number of adherent bacteria. Medians and 25%/75% percentiles were calculated, and statistical analysis was performed using the Kruskal-Wallis test and the Bonferroni-adjusted Mann-Whitney U test.
The highest values, indicating high adhesion of streptococci, were observed for filler-supplemented teeth with median relative fluorescence values ranging from 6356 to 18,770. Similar values were recorded for a double cross-linked resin tooth (6444). Significantly lowest values, ranging from 1173 to 3974, were found for unfilled PMMA acrylic resin teeth and acrylic resin teeth with an interpenetrating network (1436).
Within the limitations of this study, it can be concluded that the adhesion of Streptococcus mutans to unfilled PMMA teeth and teeth with an interpenetrating network is lower than adhesion to artificial teeth supplemented with fillers or double cross-linked acrylic resin teeth.
假牙上的菌斑可能会促使假牙性口炎以及局部假牙相邻牙龈组织中牙周疾病的发生。因此，牙科材料对菌斑附着的敏感性较低是有益的。
本研究的目的是评估常用人造牙对口腔细菌变形链球菌附着的敏感性。
通过从颊侧牙面切割标准化板制备12种不同人造牙的15个样本。在标准化尺寸（圆形样本，直径5mm，厚2mm）、抛光（砂纸，粒度1000和4000；通用抛光膏）并使用轮廓测量接触表面测量装置评估表面粗糙度后，将样本在37℃下与变形链球菌NCTC 10449悬液孵育2.5小时。使用一种 veneering复合树脂（Sinfony）作为对照。使用荧光测定法（刃天青还原）对附着细菌进行定量；相对荧光强度与附着细菌数量呈线性相关。计算中位数和25%/75%百分位数，并使用Kruskal-Wallis检验和Bonferroni校正的Mann-Whitney U检验进行统计分析。
对于添加填料的牙齿，观察到最高值，表明链球菌附着性高，中位数相对荧光值范围为6356至18770。双交联树脂牙（6444）记录到类似值。未填充的聚甲基丙烯酸甲酯（PMMA）丙烯酸树脂牙和具有互穿网络的丙烯酸树脂牙（1436）的显著最低值范围为1173至3974。
在本研究的局限性内，可以得出结论，变形链球菌对未填充的PMMA牙和具有互穿网络的牙齿的附着低于对添加填料的人造牙或双交联丙烯酸树脂牙的附着。
