通过单精子分型进行基因定位。
Genetic mapping by single sperm typing.
作者信息
Arnheim N, Li H, Cui X
机构信息
Department of Biological Sciences, University of Southern California, Los Angeles 90089-1340.
出版信息
Anim Genet. 1991;22(2):105-15. doi: 10.1111/j.1365-2052.1991.tb00652.x.
Abstract
The polymerase chain reaction makes it possible to analyse DNA sequences in a single cell and has led to a new approach for constructing genetic maps. We describe a procedure called 'sperm typing' which can accurately classify individual meiotic products as recombinant of non-recombinant. This permits the linkage relationships among DNA polymorphisms to be determined without pedigree analysis.
摘要
聚合酶链反应使分析单个细胞中的DNA序列成为可能，并带来了一种构建遗传图谱的新方法。我们描述了一种称为“精子分型”的程序，它可以准确地将单个减数分裂产物分类为重组型或非重组型。这使得无需系谱分析就能确定DNA多态性之间的连锁关系。
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