Xue Wen J, Fan Dai D, Shang Longan, Zhu Chen H, Ma Xiao X, Yu Yuan Y
Department of Chemical Engineering and Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, 710069, China.
Biotechnol Lett. 2009 Feb;31(2):221-6. doi: 10.1007/s10529-008-9852-9. Epub 2008 Oct 16.
The evolution of CO(2) in a fed-batch culture of recombinant Escherichia coli containing human-like collagen (HLC) cDNA was determined with an O(2)-enriched air supply (40%, v/v) in a 12.8 l fermentor; a maximum CO(2) concentration of 12.7% in the effluent gas was detected. The CO(2) pulse injection experiments showed that: (1) a 20% CO(2) pulse introduced in the batch cultivation phases inhibited cell growth but if introduced in the fed-batch cultivation phases slightly stimulated growth; and (2) CO(2) inhibited HLC expression only in the expression phase, where the final HLC concentration decreased by 34% under a 3 h 20% CO(2) pulse. The higher the CO(2) concentration and/or the longer the duration of the CO(2) pulse, the stronger the stimulatory or inhibitory effects.
在12.8升发酵罐中，通过供应富氧空气（40%，v/v）来测定含有类人胶原蛋白（HLC）cDNA的重组大肠杆菌补料分批培养过程中二氧化碳（CO₂）的释放情况；检测到废气中二氧化碳的最大浓度为12.7%。二氧化碳脉冲注入实验表明：（1）在分批培养阶段引入20%的二氧化碳脉冲会抑制细胞生长，但在补料分批培养阶段引入则会轻微刺激生长；（2）二氧化碳仅在表达阶段抑制HLC表达，在3小时20%二氧化碳脉冲条件下，最终HLC浓度下降了34%。二氧化碳浓度越高和/或二氧化碳脉冲持续时间越长，刺激或抑制作用越强。
