在具有不同二氧化碳脉冲的大肠杆菌培养过程中生物质和重组类人胶原蛋白的产生。

Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses.

作者信息

Xue Wen J, Fan Dai D, Shang Longan, Zhu Chen H, Ma Xiao X, Yu Yuan Y

机构信息

Department of Chemical Engineering and Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Xi'an, 710069, China.

出版信息

Biotechnol Lett. 2009 Feb;31(2):221-6. doi: 10.1007/s10529-008-9852-9. Epub 2008 Oct 16.

DOI:10.1007/s10529-008-9852-9

PMID:18923911

Abstract

The evolution of CO(2) in a fed-batch culture of recombinant Escherichia coli containing human-like collagen (HLC) cDNA was determined with an O(2)-enriched air supply (40%, v/v) in a 12.8 l fermentor; a maximum CO(2) concentration of 12.7% in the effluent gas was detected. The CO(2) pulse injection experiments showed that: (1) a 20% CO(2) pulse introduced in the batch cultivation phases inhibited cell growth but if introduced in the fed-batch cultivation phases slightly stimulated growth; and (2) CO(2) inhibited HLC expression only in the expression phase, where the final HLC concentration decreased by 34% under a 3 h 20% CO(2) pulse. The higher the CO(2) concentration and/or the longer the duration of the CO(2) pulse, the stronger the stimulatory or inhibitory effects.

摘要

在12.8升发酵罐中，通过供应富氧空气（40%，v/v）来测定含有类人胶原蛋白（HLC）cDNA的重组大肠杆菌补料分批培养过程中二氧化碳（CO₂）的释放情况；检测到废气中二氧化碳的最大浓度为12.7%。二氧化碳脉冲注入实验表明：（1）在分批培养阶段引入20%的二氧化碳脉冲会抑制细胞生长，但在补料分批培养阶段引入则会轻微刺激生长；（2）二氧化碳仅在表达阶段抑制HLC表达，在3小时20%二氧化碳脉冲条件下，最终HLC浓度下降了34%。二氧化碳浓度越高和/或二氧化碳脉冲持续时间越长，刺激或抑制作用越强。