Yao C L, Magill C W, Frederiksen R A
Department of Plant Pathology, Texas A&M University, College Station 77843.
Appl Environ Microbiol. 1991 Jul;57(7):2027-32. doi: 10.1128/aem.57.7.2027-2032.1991.
A recombinant plasmid, pMLY12-1, screened from a Peronosclerospora sorghi library hybridizes only to DNA of P. sorghi, or to DNA from leaves infected with P. sorghi, not to DNA of P. sorghi Thailand isolate, P. philippinensis, P. sacchari, or P. maydis. The terminal sequences of the 1.3-kb insert, which appears to contain mitochondrial DNA, are 85% A and T. No polymorphisms were detected when the probe was hybridized to Southern blots containing DNA from P. sorghi pathotype 1, pathotype 3, or a Botswana isolate digested with any of the eight restriction endonucleases tested. The banding patterns were the same whether DNA was extracted directly from the fungus or from infected leaves.
从高粱霜霉文库中筛选出的重组质粒pMLY12 - 1仅与高粱霜霉的DNA杂交，或与感染高粱霜霉的叶片DNA杂交，而不与高粱霜霉泰国分离株、菲律宾霜霉、甘蔗霜霉或玉米霜霉的DNA杂交。1.3kb插入片段的末端序列（似乎包含线粒体DNA）中A和T占85%。当该探针与含有经8种测试限制性内切酶中任何一种消化的高粱霜霉致病型1、致病型3或博茨瓦纳分离株的DNA的Southern杂交膜杂交时，未检测到多态性。无论DNA是直接从真菌中提取还是从感染叶片中提取，条带模式都是相同的。
