Karram Khalad, Goebbels Sandra, Schwab Markus, Jennissen Katja, Seifert Gerald, Steinhäuser Christian, Nave Klaus-Armin, Trotter Jacqueline
Molecular Cell Biology, Department of Biology, Johannes Gutenberg University of Mainz, 55128 Mainz, Germany.
Genesis. 2008 Dec;46(12):743-57. doi: 10.1002/dvg.20440.
The NG2 glycoprotein is a type I membrane protein expressed by immature cells in the developing and adult mouse. NG2+ cells of the embryonic and adult brain have been principally viewed as oligodendrocyte precursor cells but have additionally been considered a fourth glial class. They are likely to be a heterogeneous population. In order to facilitate studies on the function of NG2+ cells and to characterize these cells in situ, we generated an enhanced yellow fluorescent protein (EYFP) "knockin mouse." EYFP-expressing cells in heterozygous knockin mice expressed the NG2 protein in all regions and at all ages studied. The EYFP+ cells did not express markers of mature glia, developing or mature neurons or microglia, but expressed markers typical for immature oligodendrocyte-lineage cells. Examination of the hippocampus showed heterogeneity in the population with regard to expression of S100ss and glutamine synthetase. Furthermore, different subpopulations of NG2+ cells in the hippocampus could be recognized by their electrophysiological properties.
NG2糖蛋白是一种I型膜蛋白，由发育中和成年小鼠的未成熟细胞表达。胚胎和成年大脑中的NG2+细胞主要被视为少突胶质前体细胞，但也被认为是第四种神经胶质细胞类型。它们可能是一个异质性群体。为了便于研究NG2+细胞的功能并在原位对这些细胞进行表征，我们构建了一种增强型黄色荧光蛋白（EYFP）“敲入小鼠”。在杂合敲入小鼠中，表达EYFP的细胞在所有研究的区域和所有年龄段均表达NG2蛋白。EYFP+细胞不表达成熟神经胶质细胞、发育中或成熟神经元或小胶质细胞的标志物，但表达未成熟少突胶质细胞系细胞典型的标志物。对海马体的检查显示，该群体在S100β和谷氨酰胺合成酶的表达方面存在异质性。此外，海马体中不同亚群的NG2+细胞可通过其电生理特性识别。
