Farré C, Velasco J, Ramón F
Servei de Bioquimica Hospital Sant Joan de Deu Carretera d'Esplugas Barcelona s/n 08034 Spain.
J Automat Chem. 1991;13(5):217-20. doi: 10.1155/S1463924691000366.
The selective multitest Olympus AU-510 analyser was evaluated according to the recommendations of the Comision de Instrumentacion de la Sociedad Española de Quimica Clinica and the European Committee for Clinical Laboratory Standards. The evaluation was carried out in two stages: an examination of the analytical units and then an evaluation in routine work conditions. The operational characteristics of the system were also studied.THE FIRST STAGE INCLUDED A PHOTOMETRIC STUDY: dependent on the absorbance, the inaccuracy varies between +0.5% to -0.6% at 405 nm and from -5.6% to 10.6% at 340 nm; the imprecision ranges between -0.22% and 0.56% at 405 nm and between 0.09% and 2.74% at 340 nm. Linearity was acceptable, apart from a very low absorbance for NADH at 340 nm; and the imprecision of the serum sample pipetter was satisfactory.TWELVE SERUM ANALYTES WERE STUDIED UNDER ROUTINE CONDITIONS: glucose, urea urate, cholesterol, triglycerides, total bilirubin, creatinine, phosphate, iron, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyl transferase.The within-run imprecision (CV%) ranged from 0.67% for phosphate to 2.89% for iron and the between-run imprecision from 0.97% for total bilirubin to 7.06% for iron. There was no carryover in a study of the serum sample pipetter. Carry-over studies with the reagent and sample pipetters shows some cross contamination in the iron assay.
根据西班牙临床化学学会仪器委员会和欧洲临床实验室标准委员会的建议,对选择性多检测奥林巴斯AU - 510分析仪进行了评估。评估分两个阶段进行:首先检查分析单元,然后在常规工作条件下进行评估。还研究了该系统的操作特性。第一阶段包括一项光度学研究:吸光度不同时,在405nm处不准确度在 +0.5%至 -0.6%之间变化,在340nm处为 -5.6%至10.6%;在405nm处不精密度范围为 -0.22%至0.56%,在340nm处为0.09%至2.74%。除340nm处NADH吸光度极低外,线性度可接受;血清样品移液器的不精密度令人满意。在常规条件下研究了12种血清分析物:葡萄糖、尿素尿酸、胆固醇、甘油三酯、总胆红素、肌酐、磷酸盐、铁、天冬氨酸转氨酶、丙氨酸转氨酶和γ-谷氨酰转移酶。批内不精密度(CV%)范围从磷酸盐的0.67%到铁的2.89%,批间不精密度从总胆红素的0.97%到铁的7.06%。血清样品移液器的研究中未发现残留。试剂和样品移液器的残留研究表明铁测定中存在一些交叉污染。