Lasnier E, Mario N, Boque M C, You S N, Vaubourdolle M
Service de Biochimie A, H pital Saint-Antoine, AP-HP, Paris, France.
Clin Chem Lab Med. 2000 Oct;38(10):1043-9. doi: 10.1515/CCLM.2000.155.
The Olympus AU400 analyser (Olympus, Tokyo, Japan) is an automated chemistry instrument for turbidimetric, spectrophotometric and ion selective electrode measurements. Overall analytical performances of the AU400 and the reagents provided by Olympus were evaluated according to the French Society of Clinical Biology guidelines. Twenty parameters including specific proteins, substrates, enzyme activities and electrolytes were tested. The linearity exceeded the specifications given by the manufacturer. Within- and between-run imprecision (CV%), evaluated at two levels, was below 1.5% for ion selective electrode parameters and 3% for other analytes, except for CO2, alkaline phosphatase at low levels and magnesium. Results compared well with those obtained with the analysers routinely used in our laboratory (Behring BNII, Olympus AU800 and Beckman CX3 Delta). The usual positive interferences from lipaemia and haemoglobin on total protein measurement were observed. Creatine kinase and alkaline phosphatase assays were the subject of positive and negative interference by haemoglobin, respectively. There was a negative interference by bilirubin in the uric acid, aspartate-amino transferase, creatine kinase and lactate dehydrogenase assays and a positive interference in the calcium assay. The system was found to be very easy to use and the workstation is user-friendly.
奥林巴斯AU400分析仪（日本东京奥林巴斯公司）是一种用于比浊法、分光光度法和离子选择性电极测量的自动化化学分析仪。按照法国临床生物学协会的指南，对AU400分析仪及奥林巴斯提供的试剂的整体分析性能进行了评估。测试了包括特定蛋白质、底物、酶活性和电解质在内的20项参数。线性范围超过了制造商给出的规格。在两个水平上评估的批内和批间不精密度（CV%），对于离子选择性电极参数低于1.5%，对于其他分析物低于3%，但二氧化碳、低水平碱性磷酸酶和镁除外。结果与我们实验室常规使用的分析仪（拜耳BNII、奥林巴斯AU800和贝克曼CX3 Delta）所获得的结果相比良好。观察到脂血和血红蛋白对总蛋白测量通常存在的正干扰。肌酸激酶和碱性磷酸酶检测分别受到血红蛋白的正干扰和负干扰。胆红素在尿酸、天冬氨酸氨基转移酶、肌酸激酶和乳酸脱氢酶检测中存在负干扰，而在钙检测中存在正干扰。发现该系统非常易于使用，且工作站对用户很友好。
