Ecob-Johnston M
Acta Virol. 1977 May;21(3):256-9.
The replication of measles and SSPE viruses in enucleate BSC-1 cells was measured by plaque assay, and the synthesis of virus-specific antigen was measured by indirect immunofluorescence. Titres of infectious virus produced in enucleate cells at 24 hours post inoculation (p.i.) were consistently 100-fold less, and the number of enucleate cells containing virus antigen at 30 hours p.i. was 10-fold less, than in nucleate control cultures. Enucleate cells at this time had 30% of the protein synthetic capacity of control nucleate cells, and supported the growth of Semliki Forest virus to titres equivalent to nucleate cell production.
通过蚀斑试验测定麻疹病毒和亚急性硬化性全脑炎（SSPE）病毒在去核BSC - 1细胞中的复制情况，并通过间接免疫荧光法测定病毒特异性抗原的合成。接种后24小时（p.i.）在去核细胞中产生的感染性病毒滴度始终比有核对照培养物低100倍，接种后30小时含有病毒抗原的去核细胞数量比有核对照培养物少10倍。此时，去核细胞的蛋白质合成能力为对照有核细胞的30%，并能支持Semliki森林病毒生长至与有核细胞产生的滴度相当。
