Stroh Justin G, Petucci Christopher J, Brecker Scott J, Nogle Lisa M
Discovery Analytical Chemistry, Chemical and Screening Sciences, Wyeth Research, Collegeville, PA 19426, USA.
J Sep Sci. 2008 Dec;31(21):3698-703. doi: 10.1002/jssc.200800399.
Recent advances in accurate mass analysis are poised to allow the high-throughput production of accurate mass data on many more compounds than was previously available. It is shown that sub-ppm mass accuracy (producing elemental compositions) can be obtained on a simple TOF mass spectrometer operating in the manufacturer's standard mode. Concomitantly, there have been important technological advances in LC with respect to speed of analysis using sub-2 microm particle columns. Much of the sub-2 microm work in the literature has been under the label ultra performance LC (UPLC), however, we show that very high-speed results can be obtained using other manufacturer's pumps by using elevated column temperatures. Using elevated temperatures, HPLC peak widths on the order of 1 s can be obtained. We report the coupling of these two technologies (sub-ppm mass accuracy MS with high-speed HPLC) for the rapid analysis of compounds entering pharmaceutical libraries.
精确质量分析的最新进展有望实现比以往更多化合物的高通量精确质量数据生成。结果表明，在以制造商标准模式运行的简单飞行时间质谱仪上可获得亚百万分之一的质量精度（生成元素组成）。与此同时，在使用亚2微米颗粒柱进行分析的速度方面，液相色谱（LC）也取得了重要的技术进步。文献中许多亚2微米的工作都打着超高效液相色谱（UPLC）的旗号，然而，我们表明，通过使用其他制造商的泵并提高柱温，可以获得非常高的分析速度。使用升高的温度，可以获得约1秒的高效液相色谱（HPLC）峰宽。我们报告了这两种技术（亚百万分之一质量精度的质谱与高速HPLC）的联用，用于快速分析进入药物库的化合物。
