Sternberg Zohara, Chadha Kailash, Lieberman Alicia, Hojnacki David, Drake Allison, Zamboni Paolo, Rocco Paluch, Grazioli Erica, Weinstock-Guttman Bianca, Munschauer Frederick
Department of Neurology, Baird MS Center, Jacobs Neurological Institute, Buffalo, NY 14223 , United States.
J Neuroimmunol. 2008 Dec 15;205(1-2):142-7. doi: 10.1016/j.jneuroim.2008.09.008. Epub 2008 Oct 15.
The study is aimed to determine the role of quercetin (3,3'4',5,7-pentahydroxy flavone), alone and in combination with human interferon-beta (IFN-beta), in modulating the immune response(s) of peripheral blood mononuclear cells (PBMC) isolated from multiple sclerosis (MS) patients and from normal healthy subjects. PBMC proliferation in the presence or absence of these drugs was determined and the production of proinflammatory cytokines (IL-1beta, TNF-alpha), and the ratio of cell migration mediator MMP-9, and its inhibitor, TIMP-1 were assessed in the culture supernatants. Quercetin reduced, in a dose-dependent manner, the proliferation of PBMC and modulated the level of IL-1beta and TNF-alpha released by PBMC in the culture supernatants. Quercetin reduced the MMP-9/TIMP-1 ratio via lowering MMP-9 production. Quercetin, when combined with IFN-beta, had additive effects in modulating TNF-alpha and MMP-9. These immunomodulatory responses to quercetin were similar between MS patients and healthy control (HC) subjects.
本研究旨在确定槲皮素（3,3',4',5,7 - 五羟基黄酮）单独以及与人类β干扰素（IFN - β）联合使用时，对从多发性硬化症（MS）患者和正常健康受试者分离出的外周血单个核细胞（PBMC）免疫反应的调节作用。测定了在有或无这些药物存在时PBMC的增殖情况，并评估了培养上清液中促炎细胞因子（IL - 1β、TNF - α）的产生，以及细胞迁移介质MMP - 9与其抑制剂TIMP - 1的比例。槲皮素以剂量依赖的方式降低了PBMC的增殖，并调节了培养上清液中PBMC释放的IL - 1β和TNF - α水平。槲皮素通过降低MMP - 9的产生降低了MMP - 9/TIMP - 1的比例。槲皮素与IFN - β联合使用时，在调节TNF - α和MMP - 9方面具有相加作用。MS患者和健康对照（HC）受试者对槲皮素的这些免疫调节反应相似。
