α-1-酸性糖蛋白与甲胎蛋白联合作为肝细胞癌的一种改进诊断工具。

Combination of alpha-1-acid glycoprotein and alpha-fetoprotein as an improved diagnostic tool for hepatocellular carcinoma.

作者信息

Bachtiar Indra, Santoso Julian Mulya, Atmanegara Benny, Gani Rino Alvani, Hasan Irsan, Lesmana Laurentius Adrianto, Sulaiman Ali, Gu Jianren, Tai Susan

机构信息

Mochtar Riady Institute for Nanotechnology, Lippo Karawaci, Banten, Indonesia.

出版信息

Clin Chim Acta. 2009 Jan;399(1-2):97-101. doi: 10.1016/j.cca.2008.09.024. Epub 2008 Sep 30.

DOI:10.1016/j.cca.2008.09.024

PMID:18926809

Abstract

BACKGROUND

To evaluate the diagnostic value of alpha-1-acid glycoprotein (AAG) and the combination with alpha-fetoprotein (AFP) in hepatocellular carcinoma (HCC) patients.

METHODS

AAG was measured in serum of 65 HCC patients and 54 chronic liver diseases (CLD) patients by using proteomic approach. Sensitivity and specificity of AAG and its combination with AFP were determined and compared with AFP alone for the diagnosis of HCC.

RESULTS

The expression concentration of AAG was significantly higher in HCC patients than chronic liver disease with sensitivity (77%) and accuracy (83%). Receiver operating characteristic analysis yielded the following AUC: AFP 0.750 (CI 95% 0.663-0.837), AAG 0.907 (CI 95% 0.855-0.960) and AFP+AAG 0.943 (CI 95% 0.897-0.988). At a specificity of 90%, the combination of AFP+AAG had sensitivity 89% and accuracy 90%, which was higher than sensitivity (52.3%) and accuracy (70%) when using AFP alone.

CONCLUSION

The combination of AAG and AFP shows high sensitivity and improves the accuracy of HCC diagnosis.

摘要

背景

评估α-1-酸性糖蛋白（AAG）及其与甲胎蛋白（AFP）联合检测在肝细胞癌（HCC）患者中的诊断价值。

方法

采用蛋白质组学方法检测65例HCC患者和54例慢性肝病（CLD）患者血清中的AAG。确定AAG及其与AFP联合检测的敏感性和特异性，并与单独使用AFP诊断HCC进行比较。

结果

HCC患者中AAG的表达浓度显著高于慢性肝病患者，其敏感性为77%，准确性为83%。受试者工作特征分析得出以下曲线下面积（AUC）：AFP为0.750（95%置信区间0.663 - 0.837），AAG为0.907（95%置信区间0.855 - 0.960），AFP + AAG为0.943（95%置信区间0.897 - 0.988）。在特异性为90%时，AFP + AAG联合检测的敏感性为89%，准确性为90%，高于单独使用AFP时的敏感性（52.3%）和准确性（70%）。