Cha Sang-Wook, Tadjuidje Emmanuel, Tao Qinghua, Wylie Christopher, Heasman Janet
Division of Developmental Biology, Cincinnati Children's Research Foundation, Cincinnati, OH 45229, USA.
Development. 2008 Nov;135(22):3719-29. doi: 10.1242/dev.029025. Epub 2008 Oct 16.
Wnt signaling in development and adult tissue homeostasis requires tight regulation to prevent patterning abnormalities and tumor formation. Here, we show that the maternal Wnt antagonist Dkk1 downregulates both the canonical and non-canonical signaling that are required for the correct establishment of the axes of the Xenopus embryo. We find that the target Wnts of Dkk activity are maternal Wnt5a and Wnt11, and that both Wnts are essential for canonical and non-canonical signaling. We determine that Wnt5a and Wnt11 form a previously unrecognized complex. This work suggests a new aspect of Wnt signaling: two Wnts acting in a complex together to regulate embryonic patterning.
在发育过程和成年组织稳态中，Wnt信号传导需要严格调控，以防止模式异常和肿瘤形成。在此，我们表明母体Wnt拮抗剂Dkk1下调了非洲爪蟾胚胎轴正确建立所需的经典和非经典信号传导。我们发现Dkk活性的靶标Wnt是母体Wnt5a和Wnt11，并且这两种Wnt对于经典和非经典信号传导都是必不可少的。我们确定Wnt5a和Wnt11形成了一种先前未被识别的复合物。这项工作揭示了Wnt信号传导的一个新方面：两种Wnt共同形成复合物来调节胚胎模式形成。
