Meling G I, Clausen O P, Bergan A, Schjølberg A, Rognum T O
Institute of Forensic Medicine, National Hospital, University of Oslo, Norway.
Br J Cancer. 1991 Aug;64(2):339-44. doi: 10.1038/bjc.1991.302.
Eighty-nine fresh tissue samples from flat colonic mucosa, polypoid lesions, macroscopically evident carcinomas, and metastatic carcinomas from ten patients with longstanding ulcerative colitis (greater than or equal to 8 years duration) were analysed by DNA flow cytometry and light microscopy. Of a total of ten carcinomas found in six patients, six showed DNA aneuploidy. Three patients developed metastatic carcinomas, all with aneuploid cell populations with similar DNA indices as in the primary carcinoma. Furthermore, aneuploid cell populations with similar DNA indices often occurred, both in separate mucosa samples, as well as in mucosa and carcinoma samples, from the same patient. DNA aneuploidy was found in flat mucosa in five of the six patients with carcinoma, and in one of the four patients without carcinoma (P greater than 0.1). High grade dysplasia was found in only four of the six cases with carcinoma, indicating that high grade dysplasia is insufficient as marker for malignant development. DNA aneuploidy was found in 24% of the dysplastic mucosa samples, and in 18% of the non-dysplastic mucosa samples (n.s., both with regard to high and low grade dysplasia). Since abnormal DNA ploidy pattern was not confined to dysplastic epithelium only, DNA aneuploidy in flat mucosa may constitute an additional marker in the identification of patients at increased cancer risk who could benefit from a closer surveillance.
对来自10例患有长期溃疡性结肠炎（病程大于或等于8年）患者的89份新鲜组织样本进行分析，这些样本包括扁平结肠黏膜、息肉样病变、肉眼可见的癌组织以及转移癌组织，分析方法为DNA流式细胞术和光学显微镜检查。在6例患者中总共发现10处癌灶，其中6处显示DNA非整倍体。3例患者发生了转移癌，所有转移癌的细胞群体均为非整倍体，其DNA指数与原发癌相似。此外，具有相似DNA指数的非整倍体细胞群体经常出现，无论是在同一患者的不同黏膜样本中，还是在黏膜和癌组织样本中。在6例患有癌的患者中，有5例在扁平黏膜中发现DNA非整倍体，在4例无癌的患者中有1例发现DNA非整倍体（P>0.1）。在6例患有癌的病例中，只有4例发现高级别发育异常，这表明高级别发育异常不足以作为恶性进展的标志物。在24%的发育异常黏膜样本和18%的非发育异常黏膜样本中发现了DNA非整倍体（无论高级别还是低级别发育异常，均无统计学差异）。由于异常的DNA倍体模式不仅局限于发育异常的上皮细胞，扁平黏膜中的DNA非整倍体可能是识别癌症风险增加患者的另一个标志物，这些患者可能受益于更密切的监测。
