Zhang Rong-Li, Jiang Er-Lie, Wang Mei, Zhou Zheng, Zhai Wen-Jing, Zhai Wei-Hua, Wang Hua, Wang Zhi-Yong, Bao Yu-Shi, DU Hong, Han Ming-Zhe
Chinese Academy of Medical Sciences, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Oct;16(5):1111-5.
The study was purposed to investigate the differentiation ability of mesenchymal stem cells (MSCs) into myocardial cells in vitro. Rat bone marrow-derived MSCs were labeled and co-cultured with neonatal rat cardiomyocytes (CM) for 5 - 7 days. The expression of cell surface antigens was detected by flow cytometry, and the expression of muscle-specific marker myosin and troponin T in labeled cells was detected by immunofluorescence. The results showed that in vitro cultured MSCs expressed CD90, CD44, CD105, CD54, not expressed CD34, CD45, CD31. After co-cultured with neonatal rat CM, labeled MSCs differentiated into cardiomyocyte-like cells expressing myosin and troponin T. It is concluded that MSCs can differentiate into cardiomyocyte-like cells when co-cultured with neonatal myocardial cells in vitro. In co-culture of two kind of cells in ratio of four to one showed obvious efficacy differentiating MSCs into CMs.
本研究旨在探讨间充质干细胞（MSCs）在体外分化为心肌细胞的能力。将大鼠骨髓来源的MSCs进行标记，并与新生大鼠心肌细胞（CM）共培养5 - 7天。通过流式细胞术检测细胞表面抗原的表达，通过免疫荧光检测标记细胞中肌肉特异性标志物肌球蛋白和肌钙蛋白T的表达。结果显示，体外培养的MSCs表达CD90、CD44、CD105、CD54，不表达CD34、CD45、CD31。与新生大鼠CM共培养后，标记的MSCs分化为表达肌球蛋白和肌钙蛋白T的心肌样细胞。结论是，MSCs在体外与新生心肌细胞共培养时可分化为心肌样细胞。两种细胞以四比一的比例共培养时，MSCs向CMs分化的效果明显。
