Ozden M Y, Hasirci V N
Department of Biological Sciences, Middle East Technical University Ankara, Turkey.
Biochim Biophys Acta. 1991 Sep 2;1075(1):102-8. doi: 10.1016/0304-4165(91)90081-q.
Glucose oxidase was entrapped in small unilamellar vesicles composed of phosphatidylcholine, dicetyl phosphate and cholesterol. Prediction of the enzyme content of liposomes by calculations based on input concentrations of lipid and protein, dimensions of the lipids and the liposomes yielded one protein per vesicle. The entrapment efficiency was experimentally determined to be about 13%. On the other hand the entrapment efficiency for the small chromate ions was found to be significantly lower (0.1%). The liposomes were then coated with a polymer, poly(1,4-pyridinium diylethylene salt). It was possible to remove the lipoid material from underneath the polymer layer with various techniques. The effect of sonication and treatment with organic solvents (tested for this purpose) on enzyme activity were found to be very significant and Triton X-100 was chosen for this purpose. It was shown that the enzyme within the remaining net has 89% of its original activity.
葡萄糖氧化酶被包裹在由磷脂酰胆碱、磷酸二鲸蜡酯和胆固醇组成的小单层囊泡中。根据脂质和蛋白质的输入浓度、脂质和脂质体的尺寸通过计算预测脂质体的酶含量，得出每个囊泡含有一个蛋白质。实验测定包封率约为13%。另一方面，发现小铬酸根离子的包封率显著更低（0.1%）。然后用聚合物聚（1,4 - 吡啶二亚乙基盐）包被脂质体。采用各种技术可以从聚合物层下方去除类脂物质。发现超声处理和用有机溶剂处理（为此目的进行测试）对酶活性的影响非常显著，为此选择了Triton X - 100。结果表明，剩余网络中的酶具有其原始活性的89%。
