[使用无脱钙方法对听觉感觉器官外侧部分的超微结构研究]
[Ultra-structural study of the lateral portion of the auditory sensorial organ using a decalcification-free method].
作者信息
Méndez-Benegassi Iván, Trinidad Almudena, García-Berrocal José Ramón, Martín Cristina, Martínez-Monedero Rodrigo, Verdaguer José María, Ramírez-Camacho Rafael
机构信息
Grupo de Investigación Otológica, Servicio de Otorrinolaringología, Hospital Universitario Puerta de Hierro, Universidad Autónoma de Madrid, Madrid, España.
出版信息
Acta Otorrinolaringol Esp. 2008 Oct;59(8):377-83.
OBJECTIVE
To explain the development of a new personal technique to study the spiral ligament and stria vascularis in Guinea pig cochleae by obtaining sample tissue without decalcification and to assess its validity for electron microscopy analysis.
MATERIAL AND METHOD
Samples were taken from five female Guinea pigs weighing 200-250 g and were fixed in glutaraldehyde and paraformaldehyde for analysis of the spiral ligament and stria vascularis ultrastructure by transmission electron microscopy.
RESULTS
All of the ultrastructure components in the spiral ligament and stria vascularis could be examined without the need for decalcification.
CONCLUSIONS
Our method to obtain and analyze samples of cochlea side wall is valid, easy and faster.
目的
解释一种新的个人技术的发展，该技术通过获取未经脱钙的样本组织来研究豚鼠耳蜗的螺旋韧带和血管纹，并评估其用于电子显微镜分析的有效性。
材料与方法
从五只体重200 - 250克的雌性豚鼠身上取样，固定于戊二醛和多聚甲醛中，通过透射电子显微镜分析螺旋韧带和血管纹的超微结构。
结果
螺旋韧带和血管纹的所有超微结构成分无需脱钙即可检查。
结论
我们获取和分析耳蜗侧壁样本的方法有效、简便且快速。
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