Hougland Matthew Tyler, Gao Yonglin, Herman Laura, Ng Chin K, Lei Zhenmin, El-Mallakh Rif S
Department of Psychiatry and Behavioral Sciences, University of Louisville School of Medicine, Louisville, KY, United States.
Psychiatry Res. 2008 Nov 30;164(2):166-71. doi: 10.1016/j.pscychresns.2008.01.004. Epub 2008 Oct 19.
Intracerebroventricular (ICV) administration of ouabain to young adult rats has been suggested to model human bipolar mania. In the human condition, mania and bipolar depression are both associated with reductions in frontal cerebral metabolism. We utilized [(18)F]-fluorodeoxyglucose [(18)FDG] positron emission tomography (PET) to visualize glucose uptake in animals receiving ICV ouabain. Animals received 5 microl of 10(-)(3) M ouabain ICV, were anesthetized with isoflurane inhalation, and administered intraperitoneally with 0.5 mCi of (18)FDG. PET data were collected over 20 min 1 hour later. Additionally, the effect of lithium was examined in animals receiving lithium in their diet for 1 week before the ICV ouabain injection. Data were analyzed with IDL Virtual Machine software. Brain glucose utilization as measured by (18)FDG uptake was significantly reduced in animals receiving ICV ouabain compared with those receiving equal volumes of artificial cerebrospinal fluid. Pretreatment with lithium normalized (18)FDG uptake. These results mirror human studies.
向成年大鼠脑室内（ICV）注射哇巴因已被认为可模拟人类双相躁狂症。在人类疾病中，躁狂症和双相抑郁症均与额叶脑代谢降低有关。我们利用[（18）F] - 氟脱氧葡萄糖[（18）FDG]正电子发射断层扫描（PET）来观察接受脑室内注射哇巴因动物的葡萄糖摄取情况。动物接受5微升10（-）（3）M的哇巴因脑室内注射，通过吸入异氟烷进行麻醉，并腹腔注射0.5毫居里的（18）FDG。1小时后在20分钟内收集PET数据。此外，在脑室内注射哇巴因前1周，对饮食中添加锂的动物进行了锂效应研究。数据使用IDL虚拟机软件进行分析。与接受等量人工脑脊液的动物相比，接受脑室内注射哇巴因的动物通过（18）FDG摄取测量的脑葡萄糖利用率显著降低。锂预处理使（18）FDG摄取恢复正常。这些结果与人类研究结果相符。
