Bessos H, Murphy W G, Seghatchian M J
Edinburgh and South-East Scotland Blood Transfusion Service, Royal Infirmary Edinburgh, London, UK.
Blood Coagul Fibrinolysis. 1991 Apr;2(2):373-6. doi: 10.1097/00001721-199104000-00023.
Platelet membrane glycoprotein Ib (GPIb) is one of many GPs on the platelet membrane which contribute to the functional and morphological properties of platelets. A principal function of GPIb is its attachment to von Willebrand Factor (vWF) on injured blood vessels which leads to the adhesion of platelets to these vessels. The binding site to vWF resides on glycocalicin (GC), which is a major segment of GPIb. Glycoprotein Ib is particularly susceptible to centrifugation and storage of platelets. The assessment of GPIb status on platelets, therefore, comprises one of many traditional methods for monitoring the quality of platelets during storage. We have recently developed a novel ELISA to monitor GC levels in the supernatant of platelet concentrates (PCs) during storage. Using this ELISA we observed a progressive rise of GC in PC supernatants during storage. A recent study of citrated PCs with or without EDTA produced similar results, and showed a substantial increase of GC levels in EDTA-treated PCs. The GC ELISA could therefore be used as a novel method to monitor PCs during storage under various conditions.
血小板膜糖蛋白 Ib（GPIb）是血小板膜上众多糖蛋白之一，它对血小板的功能和形态特性有重要作用。GPIb 的一个主要功能是它与受损血管上的血管性血友病因子（vWF）结合，从而使血小板黏附到这些血管上。与 vWF 的结合位点位于糖萼蛋白（GC）上，GC 是 GPIb 的一个主要片段。糖蛋白 Ib 对血小板的离心和储存特别敏感。因此，评估血小板上的 GPIb 状态是储存期间监测血小板质量的众多传统方法之一。我们最近开发了一种新型酶联免疫吸附测定法（ELISA），用于监测储存期间血小板浓缩物（PC）上清液中的 GC 水平。使用这种 ELISA，我们观察到储存期间 PC 上清液中 GC 水平逐渐升高。最近一项关于添加或不添加乙二胺四乙酸（EDTA）的枸橼酸化 PC 的研究也得出了类似结果，并表明经 EDTA 处理的 PC 中 GC 水平大幅增加。因此，GC ELISA 可作为一种新型方法，用于在各种条件下储存期间监测 PC。
