Ozerol Elif, Bilgic Sedat, Iraz Mustafa, Cigli Ahmet, Ilhan Atilla, Akyol Omer
Department of Biochemistry, Faculty of Medicine, Inonu University, 44069, Malatya, Turkey.
Prog Neuropsychopharmacol Biol Psychiatry. 2009 Feb 1;33(1):20-4. doi: 10.1016/j.pnpbp.2008.09.024. Epub 2008 Oct 8.
Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion+erdosteine group, and (iv) erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in erdosteine group and there was a statistically significant increase, when compared with the erdosteine plus ischemia/reperfusion group. By treating the rats with erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that erdosteine reduces parameters of oxidative stress is well supported by the data.
实验研究表明，自由基在大鼠缺血/再灌注（I/R）期间的神经元损伤中起主要作用。厄多司坦是一种硫衍生物，具有促黏液运动、黏液溶解和清除自由基的特性。本研究的目的是探讨厄多司坦治疗对大鼠短期全脑缺血/再灌注损伤的影响。该研究在分为四组的Wistar大鼠上进行。（i）对照组，（ii）缺血/再灌注组，（iii）缺血/再灌注+厄多司坦组，和（iv）厄多司坦组。分析了大鼠红细胞和血浆中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性以及硫代巴比妥酸反应性物质（TBARSs）和一氧化氮（NO）水平。缺血/再灌注组的血浆NO水平显著高于其他组。与其他组相比，缺血/再灌注组血浆和红细胞中的SOD和GSH-Px活性降低，而TBARS水平升高。厄多司坦组的红细胞CAT活性较高，与厄多司坦加缺血/再灌注组相比有统计学意义的增加。通过用厄多司坦治疗大鼠，可防止内源性抗氧化酶（SOD、CAT、GSH-Px）的消耗以及TBARS和NO水平的升高。因此，本研究表明厄多司坦可降低氧化应激参数，数据对此提供了有力支持。
