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[聚丙烯酰胺凝胶电泳银染技术的改进。]

[Improvement of the silver-stained technique of polyacrylamide gel electrophoresis.].

作者信息

Liang Hong-Wei, Wang Chang-Zhong, Li Zhong, Luo Xiang-Zhong, Zou Gui-Wei

机构信息

1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China; 2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China; E-mail:

出版信息

Yi Chuan. 2008 Oct;30(10):1379-82. doi: 10.3724/sp.j.1005.2008.01379.

DOI:10.3724/sp.j.1005.2008.01379
PMID:18930901
Abstract

Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.

摘要

聚丙烯酰胺凝胶电泳广泛应用于SSR、SNP标记分析及遗传图谱构建。传统的银染方法在实验中存在耗时较长且步骤繁琐的缺点。本文对聚丙烯酰胺凝胶中DNA的银染方法进行了改进。改进后的方法显带快速，仅需约20分钟，且能获得高清晰度的图片。

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