[乳腺癌干细胞形成乳腺球的影响因素]

[Influence factors on the formation of mammospheres from breast cancer stem cells].

作者信息

Huang Ming-zhu, Zhang Feng-chun, Zhang Yan-yun

机构信息

Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China.

出版信息

Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Oct 18;40(5):500-4.

PMID:18931713

Abstract

OBJECTIVE

To investigate the influential factors on the formation of mammospheres from MCF-7, MDA-MB-231 and primary breast cancer epithelial cells, and to detect the proportion of CD44+/CD24(-/low) among them.

METHODS

MCF- 7, MDA-MB-231 and primary breast cancer epithelial cells were cultured in suspension to generate primary mammospheres. After 7 days of cultivation, the proportion of cancer stem cells was measured in cells derived from mammosphere cells or monolayer culture cells by flow cytometry.

RESULTS

MCF-7 had the highest efficiency of mammosphere formation (2.1%+/-0.3%), while MDA-MB-231 cells hardly had any mammosphere formation, and no mammosphere formation was observed in breast cancer epithelial cells. MCF-7 tended to adhere under stem cell culture conditions without B27. Flow cytometry analysis indicated that, compared with 2.0%+/-0.1% for the MCF-7 monolayer culture cells, 11.8%+/-0.3% and 8.2%+/-0.8% of the MCF-7 mammosphere cells from MCF-7 under different conditions were CD44+/CD24(-/low) (P < 0.01). The proportion of CD44+/CD24(-/low) expression in MDA-MB-231 cells and primary breast cancer epithelial cells was 92.2%+/-3.1% and 93.8%+/-2.4%, respectively.

CONCLUSION

Compared with monolayer culture cells, MCF-7 mammosphere cells contained higher proportion of breast cancer stem cells, while MDA-MB-231 and primary breast cancer epithelial cells did not. The expression of CD44+/CD24(-/low) in MDA-MB-231 and primary breast cancer epithelial cells did not correlate with mammosphere formation positively.

摘要

目的

研究MCF-7、MDA-MB-231和原发性乳腺癌上皮细胞形成乳腺球的影响因素，并检测其中CD44+/CD24(-/低)的比例。

方法

将MCF-7、MDA-MB-231和原发性乳腺癌上皮细胞进行悬浮培养以生成原代乳腺球。培养7天后，通过流式细胞术测量源自乳腺球细胞或单层培养细胞的细胞中癌干细胞的比例。

结果

MCF-7形成乳腺球的效率最高(2.1%±0.3%)，而MDA-MB-231细胞几乎不形成乳腺球，原发性乳腺癌上皮细胞未观察到乳腺球形成。在无B27的干细胞培养条件下，MCF-7易于贴壁。流式细胞术分析表明，与MCF-7单层培养细胞的2.0%±0.1%相比，不同条件下MCF-7的MCF-7乳腺球细胞中11.8%±0.3%和8.2%±0.8%为CD44+/CD24(-/低)(P<0.01)。MDA-MB-231细胞和原发性乳腺癌上皮细胞中CD44+/CD24(-/低)表达的比例分别为92.2%±3.1%和93.8%±2.4%。