Pinelis V G, Zagulova D V, Galkin A A, Baskakov M B, Markov Kh M
Biull Eksp Biol Med. 1991 Jun;111(6):579-81.
In experiments on mice resident and stimulated thioglycolate macrophages the changes in cytoplasmic Ca2+ concentration Ca2+ have been studied by the use of the fluorescent probe fura-2. PAF acether (10(-7) M) raised Ca2+ by 300-400 nM within 1 min only in the stimulated macrophages. In the resident cells this increase was much less. In the presence of 2mM EGTA, PAF raised Ca2+ to a lesser extent. This suggests that PAF causes influx of exogenous Ca2+ through the receptor-mediated channels as well as releasing Ca2+ from intracellular stores.
在对驻留和经巯基乙酸盐刺激的小鼠巨噬细胞进行的实验中，通过使用荧光探针fura - 2研究了细胞质Ca2 +浓度（Ca2 +）的变化。血小板活化因子（PAF）乙醚（10（-7）M）仅在经刺激的巨噬细胞中于1分钟内使Ca2 +升高300 - 400 nM。在驻留细胞中，这种升高要小得多。在存在2 mM乙二醇双乙醚二胺四乙酸（EGTA）的情况下，PAF使Ca2 +升高的程度较小。这表明PAF通过受体介导的通道引起外源Ca2 +内流以及从细胞内储存库释放Ca2 +。
