In field trials, eggs from two flocks of Large White turkey hens were injected with about 0.2 ml saline solution containing 600 micrograms of pyridoxine hydrochloride in order to examine its effect on hatchability. 2. Also, in an aseptic laboratory trial, eggs from Large White turkey hens were injected with 0.2 ml of saline solution and 0.2 ml of saline solution containing 600 micrograms of pyridoxine hydrochloride. 3. In field trials, hatchability of pyridoxine-injected eggs was 4.6% higher (P less than 0.05) than the control (non-injected) eggs. 4. In the aseptic laboratory trial, hatchability of pyridoxine-injected eggs was 4.2% higher (P less than 0.05) than saline-injected and control (non-injected) eggs.