RNA折叠与核糖体组装。
RNA folding and ribosome assembly.
作者信息
Woodson Sarah A
机构信息
T.C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218-2685, USA.
出版信息
Curr Opin Chem Biol. 2008 Dec;12(6):667-73. doi: 10.1016/j.cbpa.2008.09.024. Epub 2008 Oct 18.
Abstract
Ribosome synthesis is a tightly regulated process that is crucial for cell survival. Chemical footprinting, mass spectrometry, and cryo-electron microscopy are revealing how these complex cellular machines are assembled. Rapid folding of the rRNA provides a platform for protein-induced assembly of the bacterial 30S ribosome. Multiple assembly pathways increase the flexibility of the assembly process, while accessory factors and modification enzymes chaperone the late stages of assembly and control the quality of the mature subunits.
摘要
核糖体合成是一个受到严格调控的过程，对细胞存活至关重要。化学足迹法、质谱分析和冷冻电子显微镜正在揭示这些复杂的细胞机器是如何组装的。rRNA的快速折叠为蛋白质诱导的细菌30S核糖体组装提供了一个平台。多种组装途径增加了组装过程的灵活性，而辅助因子和修饰酶则在组装后期发挥伴侣作用并控制成熟亚基的质量。
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