Aslan Kadir, Geddes Chris D
Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, MD 21201, USA.
Analyst. 2008 Nov;133(11):1469-80. doi: 10.1039/b808292h. Epub 2008 Sep 18.
In this timely review, we summarize recent work on ultra-fast and sensitive bioassays based on microwave heating, and provide our current interpretation of the role of the combined use of microwave energy and plasmonic nanostructures for applications in rapid clinical and bioagent diagnostics. The incorporation of microwave heating into plasmonic nanostructure-based bioassays brings new advancements to diagnostic tests. A temperature gradient, created by the selective heating of water in the presence of plasmonic nanostructures, results in an increased mass transfer of target biomolecules towards the biorecognition partners placed on the plasmonic nanostructures, enabling diagnostic tests to be completed in less than a minute, and in some cases only a few seconds, by further microwave heating. The diagnostic tests can also be run in complex biological samples, such as human serum and whole blood.
在这篇及时的综述中，我们总结了基于微波加热的超快且灵敏生物检测的近期工作，并阐述了当前对于微波能量与等离子体纳米结构联合应用在快速临床诊断和生物制剂诊断中的作用的理解。将微波加热纳入基于等离子体纳米结构的生物检测为诊断测试带来了新进展。在等离子体纳米结构存在的情况下，通过对水的选择性加热产生的温度梯度，导致目标生物分子向置于等离子体纳米结构上的生物识别伙伴的传质增加，通过进一步微波加热，使得诊断测试能够在不到一分钟内完成，在某些情况下仅需几秒钟。这些诊断测试也可以在复杂生物样品中进行，如人血清和全血。
