Azevedo Luciane H, de Sousa Suzana C O M, Correa Luciana, de Paula Eduardo Carlos, Dagli Maria Lúcia Zaidan, Romanos Georgios, Migliari Dante A
Special Laboratory of Lasers in Dentistry (LELO), School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
Lasers Med Sci. 2009 Jul;24(4):585-90. doi: 10.1007/s10103-008-0616-5. Epub 2008 Oct 21.
The aim of this study was to compare the concentration of mast cells (MCs) in the healing process of incisions. Thirty rats were submitted to six linear incisions each, performed in the dorsal skin by carbon dioxide (CO(2)) and diode lasers, electrocautery and conventional scalpel. The animals were euthanized at intervals of 0 h, 24 h, 48 h, 72 h, 7 days and 14 days after the incisions had been made. Histological sections were obtained and stained with toluidine blue for identification of MCs, which were manually counted by conventional microscopy in 20 microscopic fields in the border of the incision, near the granulation tissue, or in the area of new collagen formation, depending on intervals. The concentration of MCs was significantly higher in the wounds made by scalpel than in those made by other techniques at 48 h and 72 h. After 72 h the number of MCs was also significantly higher after electrocautery than after incisions made by 4 W CO(2) laser. On days 7 and 14, there was no significant difference in the MC count among the different types of incisions. In summary, the MC concentration varied after different surgical incisions at early phases of wound healing. At the end of the healing process, however, there were similar MC concentrations around the incisions, suggesting that, in standard incisions in the surgical techniques studied, the wound healing process ultimately occurred in a similar pattern.
本研究的目的是比较切口愈合过程中肥大细胞（MCs）的浓度。30只大鼠每只均在背部皮肤制作6条线性切口，分别采用二氧化碳（CO₂）激光、二极管激光、电灼术和传统手术刀进行操作。在切口制作后的0小时、24小时、48小时、72小时、7天和14天等不同时间点对动物实施安乐死。获取组织学切片并用甲苯胺蓝染色以识别MCs，根据不同时间间隔，通过传统显微镜在切口边缘、靠近肉芽组织处或新胶原形成区域的20个显微镜视野中手动计数MCs。在48小时和72小时时，手术刀制作的伤口中MCs浓度显著高于其他技术制作的伤口。72小时后电灼术伤口的MCs数量也显著高于4W CO₂激光制作的切口。在第7天和第14天，不同类型切口的MC计数无显著差异。总之，在伤口愈合早期，不同手术切口后MC浓度有所不同。然而，在愈合过程结束时，切口周围的MC浓度相似，这表明在所研究的手术技术中的标准切口中，伤口愈合过程最终以相似的模式发生。
