用于蛋白质组学研究的从复苏植物粘叶旱生草中分离细胞核和提取核蛋白的实验方案。
Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for proteomic studies.
作者信息
Abdalla Kamal Omer, Thomson Jennifer Ann, Rafudeen Muhammad Suhail
机构信息
Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
出版信息
Anal Biochem. 2009 Jan 15;384(2):365-7. doi: 10.1016/j.ab.2008.09.049. Epub 2008 Oct 7.
The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps.
植物细胞核是一种重要的亚细胞器，但从植物中分离出纯净且富集的细胞核，并随后提取核蛋白用于蛋白质组学研究具有挑战性。在此，我们展示了从复苏植物粘叶旱生草中分离细胞核和提取核蛋白的方案，并对最关键步骤进行了优化和改进。
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