Ben Mansour Hedi, Barillier Daniel, Corroler David, Ghedira Kamel, Chekir-Ghedira Leila, Mosrati Ridha
Equipe de Recherche en Physico-Chimie et Biotechnologie (ERPCB-EA3914), Institut Universitaire de Technologie-Unité de Formation et de Recherche des Sciences, Unviersité de Caen-Basse Normandie, France.
Environ Toxicol Chem. 2009 Mar;28(3):489-95. doi: 10.1897/08-333.1. Epub 2008 Oct 21.
Mutagenicity of acid orange 52 (AO52) and its degradation products by Pseudomonas putida mt-2 was evaluated with the use of Salmonella Typhimurium TA102 and TA104 with and without the metabolic activation system (S9). No mutagenicity was observed in the absence of S9 and in the presence of S9 for biodegradation under shaking conditions, but it increased significantly in the presence of S9 after biodegradation under static conditions. In addition, the ability of tested compounds to induce DNA damage in vitro was evaluated with the DNA strand scission assay. The toxicity generated by the pure azo dye and the corresponding azoreduction products (4-aminobenzenesulfonic acid and N,N'-dimethyl-p-phenylenediamine) were compared. We suggest that the mutagenicity mechanism of these molecules occurs through free radical generation processes. In this study, we demonstrate that P. putida mt-2 incubated under aerobic conditions undergoes catabolism that enables it to degrade AO52 completely and, especially, to detoxify the dye mixtures.
利用鼠伤寒沙门氏菌TA102和TA104，在有和没有代谢活化系统（S9）的情况下，评估了酸性橙52（AO52）及其被恶臭假单胞菌mt-2降解产物的致突变性。在没有S9以及有S9且在振荡条件下进行生物降解时，均未观察到致突变性，但在静态条件下生物降解后，有S9时致突变性显著增加。此外，通过DNA链断裂试验评估了受试化合物在体外诱导DNA损伤的能力。比较了纯偶氮染料及其相应的偶氮还原产物（4-氨基苯磺酸和N，N'-二甲基对苯二胺）产生的毒性。我们认为这些分子的致突变机制是通过自由基生成过程发生的。在本研究中，我们证明了在有氧条件下培养的恶臭假单胞菌mt-2会进行分解代谢，使其能够完全降解AO52，尤其是使染料混合物解毒。
