Ma Zhaofeng, Li Shi, Song Yingliang, Tang Liang, Ma Dandan, Liu Baolin, Jin Yan
Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Tissue Eng Part A. 2008 Dec;14(12):2059-68. doi: 10.1089/ten.tea.2008.0021.
It was recognized that periodontal progenitor cells penetrate disintegrated Hertwig's epithelial root sheath, and contact with root dentin give rise to periodontium formation. Clinically, direct contact of the conditioned or denuded root surfaces with periodontal cells seems to be a prerequisite for periodontal regeneration. In this study, we investigated the biological effect of dentin noncollagenous proteins (DNCPs) on the human periodontal ligament stem cells (HPDLSCs) in vitro and in vivo. Chemical-conditioned root dentin (CCRD) was prepared by process of partly demineralization and deproteinization. Treated HPDLSCs with DNCPs showed increased proliferation and adhesion ability. Induced HPDLSCs presented several features of cementoblast differentiation, as indicated by morphologic changes, enhanced alkaline phosphatase (ALP) activity, increased matrix mineralization, and upregulated expression of mineralization-associated genes. Incubation of treated HPDLSC aggregate in vivo revealed that cementum-like tissues formed along the CCRD surface with fibrous tissue adjacent to or inserted into it, but untreated HPDLSCs cannot form similar structure. To our knowledge, this is the first study to apply active proteins derived from dentin with periodontal stem cells to construct periodontal structure, which may shed light on human periodontal tissue regeneration.
人们认识到，牙周祖细胞穿透崩解的赫特维希上皮根鞘，并与牙根牙本质接触，从而形成牙周组织。临床上，经过处理或裸露的牙根表面与牙周细胞的直接接触似乎是牙周再生的先决条件。在本研究中，我们在体外和体内研究了牙本质非胶原蛋白（DNCPs）对人牙周膜干细胞（HPDLSCs）的生物学效应。化学处理的牙根牙本质（CCRD）是通过部分脱矿质和脱蛋白过程制备的。用DNCPs处理的HPDLSCs显示出增殖和黏附能力增强。诱导的HPDLSCs呈现出成牙骨质细胞分化的几个特征，如形态学变化、碱性磷酸酶（ALP）活性增强、基质矿化增加以及矿化相关基因表达上调。在体内培养经处理的HPDLSC聚集体显示，沿CCRD表面形成了牙骨质样组织，并有纤维组织与之相邻或插入其中，但未经处理的HPDLSCs不能形成类似结构。据我们所知，这是第一项将源自牙本质的活性蛋白与牙周干细胞应用于构建牙周结构的研究，这可能为人类牙周组织再生提供线索。
