The effect of low energy LED red light on osteogenetic differentiation of periodontal ligament stem cell via the ERK5 signal pathway.

The purpose of this study was to examine how low-energy LED red light influences the early to middle stage of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) via the ERK5 signaling pathway.  METHODS: PDLSCs were extracted from periodontal membrane tissue using enzymatic digestion. At three time points of 7, 10, and 14 days after irradiation with 5J/cm LED red light, the expression levels of early to middle-stage osteogenic-related genes ALP, Col-1, BSP, and OPN were detected by real-time fluorescence quantitative PCR(qRT-PCR) in both control and osteogenesis experimental groups. The addition of BIX02189 could block the ERK5 signaling pathway. Under irradiation with 5J/cm LED red light, the expression levels of the ERK5 gene, related proteins ERK5, p-ERK5, as well as early to middle-stage osteogenic-related genes ALP, Col-1, BSP, and OPN were detected by qRT-PCR and Western blot in the osteogenic medium group and the osteogenic medium + BIX02189 group.  RESULTS: Both low-energy LED red light and osteogenic medium could induce osteogenesis and differentiation of PDLSCs, upregulating the expression of ALP, Col-1, BSP, and OPN genes in PDLSCs. Their combination also produced a synergistic effect. Moreover, the ERK5 signaling pathway participated in the promoting effect of LED red light on the early to middle-stage osteogenic differentiation of PDLSCs, indicating a positive role of LED red light in this process.  CONCLUSIONS: The ERK5 signaling pathway can mediate the promotion of early to middle-stage osteogenic differentiation of PDLSCs by low-energy LED red light.